RIULS

Repository of Iasi University of Life Sciences, ROMANIA

Welcome to RIULS!

The mission of the University’s institutional repository is to manage, preserve and disseminate, in open access, the academic and institutional output of the university’s academic community. It also aims to promote values and personalities belonging to Romanian agricultural science, by storing digitized versions of rare books and manuscripts from its collections.

The repository is part of the international movement known as open science, which promotes free access to scientific literature. This translates into maximized impact of the work carried out by researchers, contribution to improving access to knowledge, increased visibility of the institution through the works of its authors and improvement of its search engine ranking.

All documents deposited in the repository receive a unique and persistent identifier called a Handle, thus guaranteeing perpetual access to scientific output through permanent links.

RIULS Repository of Iași University of Life Sciences is indexed in Duraspace, OpenDOAR,CORE,Google Scholar,BASE (Bielefeld Academic Search Engine) and ROAR (Registry of Open Access Repositories), global Directories of Open Access Repositories.

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Recent Submissions

  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    Addisonian Crisis Mimicking Acute Kidney Injury in Dogs: A Retrospective Study of 34 Dogs Diagnosed with Acute Kidney Injury in Romania
    (MDPI, 2026-01-14) Roșca, Ștefania; Solcan, Gheorghe; Moroz, Mihail; Ștefănescu, Raluca-Adriana; Levința, Alina; Pașca, Paula-Maria
    Primary hypoadrenocorticism (Addison’s disease) is an uncommon but potentially life-threatening endocrine disorder in dogs. Affected animals may present with clinicopathological features mimicking acute kidney injury (AKI). The challenge in diagnosing hypoadrenocorticism arises from its highly heterogeneous and non-specific clinical presentation, including acute kidney injury (AKI). This retrospective observational study aimed to evaluate dogs presenting with AKI and to identify cases in which primary hypoadrenocorticism was the underlying etiology. Thirty-four dogs diagnosed with acute kidney injury were evaluated at the Clinical Hospital for Companion Animals of the “Ion Ionescu de la Brad” University of Life Sciences, Iași, Romania, among which three (8.8%) were endocrinologically confirmed to have primary hypoadrenocorticism. The evaluation protocol included a complete clinical examination, hematological, biochemical, and hormonal investigations, urinalysis, abdominal ultrasonography, and an ACTH stimulation test. These dogs exhibited hyponatremia, hyperkalemia, a reduced sodium-to-potassium ratio, and azotemia at admission, closely resembling intrinsic AKI. Following fluid therapy and hormone replacement, rapid normalization of electrolyte and renal parameters was observed. These findings support hypovolemia and electrolyte imbalance as the primary mechanisms underlying reversible prerenal azotemia in these cases. If not diagnosed early, this condition has a significant risk of progressing to acute tubular necrosis. The findings highlight the need for careful differentiation between primary AKI and renal dysfunction secondary to Addison’s disease, as well as the importance of promptly initiating hormone replacement therapy. In conclusion, hypoadrenocorticism should be considered in dogs presenting with AKI and electrolyte imbalance. Early endocrine evaluation and prompt initiation of targeted therapy are essential to avoiding misdiagnosis and optimizing clinical outcomes.
  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    Fungal Contamination of Dairy Feed and Major Mycotoxin Transfer: A Risk Evaluation for Animal Exposure and Health
    (MDPI, 2026-01-13) Poroșnicu, Ioana; Ailincăi, Luminița-Iuliana; Davidescu, Mădălina-Alexandra; Mareș, Mihai
    This study was focused on the assessment of fungal occurrence, mycotoxin dynamics, aflatoxin carry-over, and associated biochemical responses in dairy cattle. Moisture emerged as the dominant factor for fungal communities, promoting the co-proliferation of fungal genera adapted to high water activity conditions (aw > 0.90) and antagonism against xerotolerant and xerophilic species. Aspergillus spp. dominated dry substrates (aw < 0.75), Fusarium spp. showed strong positive associations with high-moisture matrices (aw > 0.90), and Penicillium spp. exhibited intermediate, substrate-dependent behavior. Mycotoxin levels fluctuated non-linearly, independently of fungal counts: ochratoxin A (OTA) concentrations in corn silage increased from approximately 12 μg/kg at the onset of the ensiling period to >240 μg/kg at silo opening, indicating dynamic mycotoxin accumulation during storage, while zearalenone (ZEA) oscillated from 40 to 170 µg/kg. Despite the variation in total aflatoxins (AFLA-T) across feed matrices, aflatoxin M1 (AFM1) in milk remained low (0.0020–0.0093 μg/kg), confirming limited carry-over. Serum biochemical parameters—alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), total bilirubin (BIL-T), total protein (PROT-T)—remained within physiological limits, yet multivariate analyses revealed metabolic modulation linked to aflatoxin exposure. AFM1 explained >7% of the variance in serum biochemical profiles according to PERMANOVA (p = 0.002), showed significant MANOVA effect (Pillai = 0.198), and displayed a significant canonical association (p < 10−13). Linear discriminant analysis further separated Normal vs. Borderline hepatic profiles, indicating subclinical physiological adaptation to chronic low-dose exposure.
  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    Update on direct embryo transfer in sheep: hatched blastocysts increase conception rates
    (Frontiers Media, 2026-02-03) Ciornei, Ștefan-Gregore
    This article presents an overview of the update on direct embryo transfer using hatched blastocysts. Recent research has observed differences in conception rates in sheep following surgical transfer. All sessions included in the study followed the same standard protocol, resulting in the creation of two groups, with the control group (CG) being the one in which blastocysts were transferred, and the experimental group (EG) received hatched blastocysts. Embryos were obtained from meat sheep through in vivo derived (IVD) and transferred to crossbred sheep synchronized with very obvious corpus luteum (CL) on at least one of the ovaries (84.21%). Thus, a retrospective study highlights the clear success of embryo recipients who also received hatched blastocysts (code 9.1) compared to recipients with blastocysts only (code 6.1, 7.1). The embryo recovery rate at 6.5 days, determined by laparoscopic uterine flushing, was 84.3%. In terms of the quality of the embryos obtained, over 74.5% were transferable (not statistically significant, 76.6% in Suffolk and 72.3% in Ille de France), and over 12% of the embryos hatched. The study found that the pregnancy rate in recipient ewes receiving code 9.1 embryos (expanded blastocysts) through direct IVD transfer during the breeding season was 86.9%. These findings, when compared to previous research, highlight the potential for further exploration and innovation in this area. Nonetheless, it is important to note that there is a scarcity of literature addressing the direct transfer of IVD embryos with expanded blastocysts.
  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    Preliminary Biochemical, Physiological, and Yield Responses of Sweet Corn to Organic and Chemical Fertilization Across Genotypes
    (MDPI, 2026-01-26) Nicorici, Petru-Liviu; Abdelnaby Elhawary, Saad Masood; Ordóñez-Díaz, Jose Luis; Sanchez-Parra, Mónica; Rădeanu, Georgiana; Caruso, Gianluca; Moreno-Rojas, Jose Manuel; Rusu, Oana-Raluca; Roșca, Mihaela; Stoleru,Vasile
    Sweet corn (Zea mays var. saccharata) is a widely cultivated crop valued for its sweet flavor and high nutritional content. Over the past decade, the area devoted to sweet corn grain production has increased substantially, driven by both its nutritional qualities and its economic value. In this context, we aimed to evaluate the impact of three genotypes (Royalty F1, Hardy F1 and Deliciosul de Bacau,) under two fertilization types (chemical and organic) compared with a control version on yield, biometrical, biochemical, and quality parameters. This research was carried out between 2022 and 2023 at an experimental station situated in the North-East region of Romania. The results revealed significant influences of cultivar, fertilization method, and the interaction between these two experimental factors on most of the analyzed indicators. Regardless of the fertilization type, the genotype Hardy F1 showed higher levels of photosynthetic activity, polyphenols (2.22 mg/g d.w.) and sucrose (6.7 g/100 g d.w.), leading to greater yield (13,995 kg/ha) than that of Deliciosul de Bacau and Royalty F1. Research on fertilization has shown that sweet corn grains under an organic method have higher levels of lycopene, chlorophyll a, chlorophyll b, total phenolic content (TPC), and fructose. In contrast, chemical fertilization more effectively supported growth, photosynthetic activity, yield, and the content of antioxidants and tannins. Regarding the combined influence of these factors, most of the nutritional characteristics of Royalty F1 were enhanced by organic fertilization, whereas those of the Hardy F1 genotype were improved by chemical fertilization. These findings provide practical guidance for selecting appropriate genotype–fertilization combinations to optimize the yield and nutritional quality of sweet corn and highlight key priorities for further research on sustainable fertilization strategies under climate change conditions.
  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    SSR-Based Genetic Diversity Assessment Among Varieties Conserved in a Romanian Grapevine (Vitis vinifera L.) Collection
    (MDPI, 2026-03-05) Hârța, Monica; Clapa, Doina; De Lorenzis, Gabriella; Colibaba, Lucia-Cintia; Rotaru, Liliana
    The present study highlights the genetic relationships among 52 grapevine varieties, including traditional and newly developed Romanian varieties and international reference cultivars, held in a small didactic ampelographic collection in Iaşi, Romania, and their genetic diversity, assessed using multivariate analysis. Twelve nuclear simple sequence repeat (SSR) markers, including nine OIV-standard descriptors, were used for genetic profiling. A total of 102 alleles were identified, with an average of 8.5 alleles per locus. The mean polymorphic information content (PIC) of 0.779 confirmed the high discriminatory power of the chosen markers. NJ dendrogram and PCoA yielded mostly similar results but did not clearly differentiate genotypes based on the selected criteria for genotype comparison (usage or historical status). STRUCTURE analysis assigned genotypes to SSR-group 1 (23.07%) and SSR-group 2 (34.61%) under K = 2 and a Q-value threshold of 0.85. The high proportion of admixed genotypes (42.32%) may reflect complex pedigrees and the migration of grapevine varieties across a wider territory surrounding Romania. The present research may serve as a starting point for future studies in Romania on the genetic structure and parental analysis of grapevine varieties held in small didactic collections, aiming to characterize and hold valuable grapevine varieties under secure conditions for future generations.