RIULS

Repository of Iasi University of Life Sciences, ROMANIA

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The mission of the University’s institutional repository is to manage, preserve and disseminate, in open access, the academic and institutional output of the university’s academic community. It also aims to promote values and personalities belonging to Romanian agricultural science, by storing digitized versions of rare books and manuscripts from its collections.

The repository is part of the international movement known as open science, which promotes free access to scientific literature. This translates into maximized impact of the work carried out by researchers, contribution to improving access to knowledge, increased visibility of the institution through the works of its authors and improvement of its search engine ranking.

All documents deposited in the repository receive a unique and persistent identifier called a Handle, thus guaranteeing perpetual access to scientific output through permanent links.

RIULS Repository of Iași University of Life Sciences is indexed in Duraspace, OpenDOAR,CORE,Google Scholar,BASE (Bielefeld Academic Search Engine) and ROAR (Registry of Open Access Repositories), global Directories of Open Access Repositories.

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Recent Submissions

  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    Milk Quality Dynamics in Romanian Black Spotted and Romanian Spotted Cattle Breeds Under Heat Stress
    (MDPI, 2026-01-21) Amariții (Pădurariu), Gabriela; Pânzaru, Claudia; Maciuc, Vasile
    Milk production and quality are increasingly affected worldwide by rising ambient temperatures associated with climate change, with heat stress (HS) representing one of the major environmental challenges for dairy cattle. HS alters physiological and metabolic processes, leading to significant changes in milk composition, particularly in regions exposed to prolonged summer heat. The Temperature–Humidity Index (THI) is widely used to assess the degree of thermal discomfort and its impact on dairy performance. This study investigated the effects of heat stress on milk quality parameters in a dairy herd managed under identical conditions, comprising Romanian Black Spotted (RBS, Holstein strain) and Romanian Spotted (RS, Simmental strain) cows. Descriptive statistics were performed using the SAVC for Windows program, while differences between means were evaluated using the t-test in GraphPad Prism 9. Milk quality traits were significantly affected when THI values exceeded 73, with a consistent decline observed from early summer onwards. In the RBS breed, milk protein content decreased significantly compared with spring values, reaching 3.25% (p < 0.0001) in 2023 and 3.35% (p < 0.01) in 2024. Similar trends were recorded in the RS breed, with minimum protein values of 3.10% (p < 0.0001) and 3.19% (p < 0.0001). Fat content, casein concentration, and milk urea levels also showed highly significant HS-related changes (p < 0.0001). Overall, heat stress negatively affected milk quality, while the RS breed appears less affected under the studied conditions than the RBS breed.
  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    The Influence of Farnesol and Tyrosol on Candida spp. Virulence Traits
    (MDPI, 2024-12-31) Ciurea, Cristina Nicoleta; Mare, Anca Delia; Mareș, Mihai; Toma, Felicia; Kosovski, Irina-Bianca; Cighir, Anca; Man, Adrian
    Introduction: Intercellular communication helps regulate the cell density and the virulence traits in yeasts and bacteria. The study aims to identify the effects of quorum sensing molecules (QSMs) like farnesol and tyrosol on Candida spp. virulence traits. Methods: The effects of farnesol and tyrosol were studied on the growth rate of Candida albicans, Candida parapsilosis, Candida krusei, Candida auris, and Candida guilliermondii at different time points, on a 48 hours incubation period. The growth rate was assessed spectrophotometrically. The biofilm formation abilities of Candida spp. were assessed by crystal violet staining technique. Moreover, the expression of C. albicans virulence genes (ALS3, HSP70, SAP2) was analyzed as a response to 100 µM farnesol and tyrosol, by RT-PCR. Results: Generally, farnesol was found to inhibit the growth rate and biofilm formation mostly in non-albicans species, while tyrosol exerted a non-consistent response on the different Candida species. The expression of ALS3 and HSP70 in C. albicans was upregulated by the QSMs. Conclusions: Both farnesol and tyrosol are involved in the regulation of Candida spp. virulence mechanisms, dependent on the used concentration and exposure time and in a species-dependent manner
  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    Evaluating the Use of Grape Pomace in Cyprinus carpio Nutrition: Effects on Growth, Biochemistry, Meat Quality, Microbiota, and Oxidative Status
    (MDPI, 2024-06-07) Barbacariu, Cristian-Alin; Dîrvariu, Lenuța; Șerban, Dana Andreea; Rîmbu, Cristina-Mihaela; Horhogea, Cristina-Elena; Dumitru, Gabriela; Todirașcu-Ciornea, Elena; Lungoci, Constantin; Burducea, Marian
    This study investigated the effects of incorporating grape pomace (GP) into fish diets on the growth performance, physiological parameters, and biochemical composition of carp (Cyprinus carpio L.). A total of 180 carp, with an average initial weight of 65 g, were reared in a recirculating aquaculture system (RAS). They were divided into a control group (C), fed with a diet containing 0% GP, and two experimental groups (V1 and V2), fed with diets containing 5% and 10% GP, respectively. The experiment lasted for eight weeks. Results revealed that most growth parameters were not significantly affected by GP inclusion, except for a notable difference in the hepatosomatic index (HSI), indicating an impact on liver size relative to body weight. The biochemical analysis of carp meat showed significant differences in moisture, protein, fat, collagen, and salt content between the control and experimental groups. Furthermore, the oxidative status assessment indicated that GP supplementation modulates oxidative stress and lipid peroxidation pathways in carp, enhancing their antioxidant defenses and overall health. Microbiological examination of the carp intestinal content showed that GP inclusion in fish diets influenced microbial parameters, particularly affecting the abundance of aerobic germs and Enterobacteriaceae.
  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    An Assessment ofWest Nile and Usutu Viruses’ Seroprevalence in Hospitalized Patients: A Preliminary Study on Flavivirus Exposure in Eastern Romania
    (MDPI, 2024-01-31) Crivei, Luciana-Alexandra; Vată, Andrei; Teodor, Dănuț; Porea, Daniela; Cozma, Andreea-Paula; Aniță, Adriana; Oșlobanu, Luanda-Elena; Moroșan, Șerban; Savuța, Gheorghe
    WNV and USUV are closely related epornitic flaviviruses transmitted by Culex mosquitoes which can cause febrile and neurodegenerative disease in humans. The impact of both viruses on public health has increased in the recent decades. Aim: The aim of the study was to evaluate the seroprevalence of WNV and USUV in hospitalized patients from eastern Romania who did not show symptoms corresponding to the case definition. Methods: Human blood samples from the hospitalized patients were collected in 2015 and from April to September 2019 in Iasi County, Romania. The samples were screened by ELISA for anti-WNV IgG, IgM, and anti-USUV IgG antibodies. Results: A cumulative seroprevalence of 3.4% was recorded for anti-WNV IgG antibodies and 9.1% for anti-WNV IgM. No sample was positive for anti-USUV antibodies. Conclusion: The cumulative seroprevalence observed provides support for the consideration of WNV as being endemic in the east of Romania. The absence of anti-USUV antibodies may be related to cross-reactivity and cohort size, thus, USUV should be considered in clinical practice and become an objective for active surveillance in Romania.
  • listelement.badge.dso-type Item , listelement.badge.access-status Open Access ,
    Bacteriological load in Holstein Friesian cows with dystocia
    (Frontiers Media, 2024-12-11) Nechifor, Florin; Ciornei, Ștefan-Gregore; Roșca, Petru
    The research took place on a farm in North-Eastern Romania with Holstein Friesian cows aged between 3 and 9 years. Bacteriological investigations were carried out throughout the year 2023, on a total of 35 cows, including 25 multiparous cows and 10 primiparous cows, 23 cows had eutocic parturitions and 12 cows had dystocic parturitions, during the first 3 weeks postpartum. In the case of dystocic parturition, biological samples yielded isolates including 9.3% strains classified as Staphylococcus, 8.1% strains of Escherichia coli, 4.1% strains of Arcanobacterium, and 2.3% strains of Klebsiella. Other bacterial types were identified in lesser proportions. In the case of eutocic parturition, Escherichia coli was most frequently isolated: 18.6% of the isolated bacterial strains, followed by 5.8% Pseudomonas spp., 4.6% Enterococcus spp., 4.6% Streptococcus species, 3.5% Staphylococcus spp., and Corynebacterium spp., and 2.3% Arcanobacterium spp. It is noted that on the 7th day of puerperium, the mean value of colony-forming units per milliliter (CFU/mL) was 74 × 104 CFU/mL in normal calving cows compared to 29 × 106 CFU/mL in cows with dystocia. The total number of recorded germs increases significantly during the first 14 days postpartum in all cases, higher levels being shown in cows with dystocic calving. For statistical analysis, the independent t-test (p < 0.05) was made by using the SPSS 16 software. The object of the research is the bacteriological load in Holstein Friesian cows depending on the type of eutocic parturition/dystocia.