Browsing by Author "Marc Zarcula, Simona"

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  • Antioxidant effect of vitamin C on porcine oocytes maturated in vitro 
    Tulcan, Camelia; Cernescu, Horia; Huțu, Ioan; Boldura, Oana-Maria; Otavă, Gabriel; Furcă, Oana; Mircu, Călin; Marc Zarcula, Simona (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi, 2019)
    Cells are vulnerable to oxidative stress during in vitro culture systems. The objective of the present study was to determine the effect of vitamin C addition in in vitro culture media on porcine oocytes maturation rate ...
  • Assessment of some mitochondrial function based on gene expression in sow oocyte cultured in vitro 
    Spătaru, Ioana-Irina; Boldura, Oana-Maria; Marc Zarcula, Simona; Otavă, Gabriel; Torda, Iuliu; Tudor, Beatrice Ana-Maria; Huțu, Ioan; Lungu, Bianca-Cornelia; Georgescu, Ovidiu-Ionuț; Mircu, Călin ("Ion Ionescu de la Brad" Iași University of Life Sciences, 2022)
    Oocyte maturation represents the highlight of developmental process in the ovarian follicle. It entirely depends on mitochondrial ability to ensure energy supplies. Beside ATP production, mitochondria is involved in ...
  • BCL2 and bax gene expression in cumulus-oocytes complexes in cow 
    Boldura, Oana-Maria; Nica, Nicoleta; Milovanov, Cornelia; Popescu, Sorina; Marc Zarcula, Simona; Bonca, Gheorghe; Mircu, Călin; Huțu, Ioan; Tulcan, Camelia (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016)
    In vitro maturation (IVM) is the first and the most important step for IVF technique because the success of in vitro embryos production highly depends on oocyte quality. In the present study it was aimed to identify the ...
  • Boar freeze-dried semen evaluation using Spermac staining 
    Tulcan, Camelia; Cernescu, Horia; Bonca, Gheorghe; Huțu, Ioan; Boldura, Oana-Maria; Otavă, Gabriel; Mircu, Călin; Marc Zarcula, Simona (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2019)
    Sperm freeze-drying is a new and alternative method to preserve male gametes in refrigeration or at room temperature. In order to protect sperm integrity special protection is required. The aim of our research was to examine ...
  • Boar freeze-dried semen in medium with antioxidants evaluated based on DNA integrity after a long-time preservation 
    Torda, Iuliu; Marc Zarcula, Simona; Tulcan, Camelia; Cernescu, Horia; Boldura, Oana-Maria; Huțu, Ioan; Otavă, Gabriel; Jurju, Diana; Tripon, Roberta; Mircu, Călin (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2020)
    Sperm freeze-drying is considered an alternative method to preserve male gametes in refrigeration or at room temperature condition. In order to preserve sperm integrity special protection is required. The aim of our research ...
  • Bovine and swine parthenots generating through electrical stimultion of the oocytes 
    Mircu, Călin; Cernescu, Horia; Ungureanu, Georgiana; Rațiu, Ana-Maria; Otavă, Gabriel; Boldura, Oana-Maria; Huțu, Ioan; Tulcan, Camelia; Keller, Thomas; Marc Zarcula, Simona (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi, 2018)
    Electrical stimulation is an alternative to chemical activation to induce 𝐶𝐶𝑎𝑎2+ influx, responsible for the formation of pores in the cellular membrane. In order to activate the oocytes, electrical stimulation (E.S.) ...
  • Collection of blastomeres in order to establish sex and isolate genetic material-review 
    Torda, Iuliu; Marc Zarcula, Simona; Brzoska, Hortensja; Spătaru, Ioana-Irina; Boldura, Oana-Maria; Tudor, George; Lungu, Bianca-Cornelia; Tudor, Beatrice Ana-Maria; Georgescu, Ovidiu-Ionuț; Huțu, Ioan; Mircu, Călin ("Ion Ionescu de la Brad" Iași University of Life Sciences, 2021)
    Globally, limited access to food needs in relation to meat or milk production has required the establishment of the sex of offspring from the embryonic stage. While the meat industry uses males, the dairy industry relies ...
  • Comparative boar and bull semen evaluation after percoll treatment 
    Godja, Gabriel; Marc Zarcula, Simona; Cernescu, Horia; Mircu, Călin; Dobre, Medana; Ardelean, V.; Otavă, Gabriel; Tulcan, Camelia; Patraș, Irina; Huțu, Ioan (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016)
    There are numerous methods used in IVF both in bovine and pig for sperm separation and Percoll gradient is one of them. After using Percoll gradient spermatic parameters were as following: motility increased to 78.33% in ...
  • Effect of rozmarinic acid supplementation on in vitro maturation of bovine oocytes 
    Marc Zarcula, Simona; Tulcan, Camelia; Boldura, Oana-Maria; Solonar, A.; Otavă, Gabriel; Godja, Gabriel; Huțu, Ioan; Mircu, Călin (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2017)
    Antioxidants supplementation of in vitro culture media exerts the key role to reduce the effects of reactive oxidative species produced during assisted reproduction technique. The objective of the study was to determine ...
  • Effects of antioxidant treatment on cell differentiation in rabbit embryos 
    Torda, Iuliu; Spătaru, Ioana-Irina; Boldura, Oana-Maria; Lungu, Bianca-Cornelia; Marc Zarcula, Simona; Bratu, Daniel; Otavă, Gabriel; Huțu, Ioan; Mircu, Călin ("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023)
    The antioxidant coenzyme Q10 can influence the expression of genes involved in apoptosis and energy metabolism of oocytes and quercetin can improve oocyte maturation and early embryonic development. In this study, the ...
  • Evaluation of sows oocytes viability through Trypan Blue staining after vitrification 
    Marc Zarcula, Simona; Mircu, Călin; Crețan, Nicoleta; Otavă, Gabriel; Tulcan, Camelia; Huțu, Ioan (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi, 2017)
    Along sperm and embryo cryopreservation that are used routinely also in animal assisted reproduction, studying are done also on animal oocyte cryopreservation in order to find the best conditions to preserve their viability. ...
  • Generating bovine embryos through ICSI 
    Keller, Thomas; Marc Zarcula, Simona; Cernescu, Horia; Tulcan, Camelia; Huțu, Ioan; Otavă, Gabriel; Rațiu, Ana-Maria; Ungureanu, Georgiana; Mircu, Călin (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi, 2017)
    Through ICSI, competition between sperms and also sperm-oocyte interaction are avoided thus ICSI proving reliable when sperm is not suitable for IVF. In bovine, the limiting step is represented by low rate of sperm head ...
  • In vitro bisphenol A effect on TFAM and SIRT1 gene expression in porcine oocyte mitochondria 
    Spătaru, Ioana-Irina; Boldura, Oana-Maria; Lungu, Bianca-Cornelia; Marc Zarcula, Simona; Torda, Iuliu; Daniel, Bratu; Otavă, Gabriel; Huțu, Ioan; Mircu, Călin ("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023)
    Mitochondria are the main cellular organelle responsible for energy production, having an essential role in maintaining cellular homeostasis. In this study, the gene expression of TFAM (Transcription Factor A Mitochondrial) ...
  • In vitro bovine embryos evaluation based on OCT4, SOX2, IGF1R and IGF2R expression level 
    Marc Zarcula, Simona; Mircu, Călin; Tulcan, Camelia; Bonca, Gheorghe; Huțu, Ioan; Otavă, Gabriel; Torda, Iuliu; Georgescu, Ovidiu-Ionuț; Andonii, Cristina; Lungu, Bianca-Cornelia; Boldura, Oana-Maria (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2020)
    In vitro production of bovine embryos comprises a lot of factors that can influence the successful of this technique, oxidative stress being one of them. These factors can influence the evolution of important development ...
  • Polimorphism screening using a vntr molecular marker system in romanian resident bison individuals 
    Boldura, Oana-Maria; Marc Zarcula, Simona; Popa, Gabriela-Irina; Popescu, Sorina; Tulcan, Camelia; Huțu, Ioan; Bonca, Gheorghe; Milovanov, Cornelia; Mircu, Călin (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2015)
    Genomic DNA isolated from the blood sampled from 14 individuals of Bison bonasus was used in order to obtain a molecular fingerprint and based on that, to obtain a dendrogram exhibiting the phylogentic relationship among ...
  • Pronuclei formation subsequent to intracytoplasmic sperm injection in bovine 
    Godja, Gabriel; Rațiu, Ana-Maria; Ungureanu, Georgiana; Keller, Thomas; Marc Zarcula, Simona; Ciobota, Andreea; Otavă, Gabriel; Tulcan, Camelia; Huțu, Ioan; Mircu, Călin (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016)
    ICSI represents the top of the range of the assembly protocols of assisted reproduction which presumes the existence of sophisticated equipments, prior acquired routine in preparing the oocytes and sperm and a very good ...
  • Quality evaluation of freeze-dried ammodytes ammodytes venom by CGE on-a chip with laser induced fluorescence detection 
    Tulcan, Camelia; Tudor, George; Boldura, Oana-Maria; Ahmadi, Mirela; Mircu, Călin; Huțu, Ioan; Milovanov, Cornelia; Sărăndan, Mihai; Marc Zarcula, Simona (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2015)
    Our study represents the first attempt to develop a protocol for evaluating the quality freeze- dried venom by using current proteomics analysis techniques The research carried out represents the initial step in the ...
  • Rabbit embryo generation through various protocols 
    Torda, Iuliu; Spătaru, Ioana-Irina; Huțu, Ioan; Marc Zarcula, Simona; Boldura, Oana-Maria; Otavă, Gabriel; Tudor, Beatrice Ana-Maria; Lungu, Bianca-Cornelia; Georgescu, Ovidiu-Ionuț; Mircu, Călin ("Ion Ionescu de la Brad" Iași University of Life Sciences, 2022)
    In rabbit ovulation is induced by mating stimulus. The length of ovulation influences the embryo developmental stages and their localization in various segments of genital tract during first 72-84 hours post mating. The ...
  • Retrospective analysis on somatic cells count trend in Staphylococcus aureus cows' mastitis 
    Huțu, Ioan; Matiuți, Marcel; Tulcan, Camelia; Marc Zarcula, Simona; Lungu, Bianca-Cornelia; Mircu, Călin (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2019)
    cells count (SCC) of two successive samplings. The analysis was performed to identify the associations between SCC trend with retrospective and transversal milk quality indicators, and mastitis. From a BIOAMR database, 28 ...
  • Vaginal smear, progesterone levels, and ultrasound examination of the ovaries as methods of determining the moment of ovulation in bitches comparative study 
    Otavă, Gabriel; Mircu, Călin; Igna, Violeta; Marc Zarcula, Simona; Lo Presti, D. (”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi, 2017)
    Researches in the scientific literature reveal that the study of vaginal citology and interpretation of progesterone values do not represent certain methods to determine the ovulation time. 6 different breed have been ...