Effects of gamma-irradiation on the mycelia of some pleurotus eryngiistrains grown in vitro

Abstract

This work focuses on evaluating the effects of applying low doses of Gamma radiation on the mycelia of some Pleurotus eryngii (King Oyster) strains.Mycelia grown on agarized media PDA and MEA were irradiated with doses ranging from 50 to 1500 Gy. Subcultures were obtained by transferring irradiated and non-irradiated mycelia to fresh PDA, MEA solid media and PDB, MEB liquid media (submerged culture). The cultural characteristics and growth rates of irradiated and non-irradiated subcultured mycelia were verified –after the first passage.Higher doses caused the mycelia to slow down and reduce the amount of biomass obtained.The dose of 200 Gy applied to the mycelia of the three strains of P.eryngii verified, in some cases, resulted better than the lower doses 50 Gy, 100 Gy, 150 Gy.