Cojocariu, Laurian-CristianPetrovici, AdrianaIlie, Ovidiu-DumitruAniță, Adriana-ElenaAniță, Dragoș-ConstantinSavuța, GheorgheRadu Rusu, Răzvan-Mihail2026-06-162026-06-1620251454-7406https://repository.iuls.ro/handle/20.500.12811/6644In recent years, biostatistics has emerged as an indispensable tool in biomedical research, playing a very important role in the design, analysis, and interpretation of data from biological and medical studies. Biostatistics encompasses a diverse range of statistical methodologies adapted to address the unique challenges presented by complex biological systems. This paper aims to explore and contribute to the further development of biostatistics by deepening into the difference between statistical analysis tests. The general purpose of this paper is to compare different tests for the analysis of the differences between the means of the control group and the experimental groups, in order to identify the optimal biostatistical test, correlated with the study and typology of the collected data. To perform these statistical tests and subsequently compare them, data were collected from a total of 60 BALB/c mice divided into 3 groups with equal numbers of individuals (one control group and two experimental groups) in whose food was introduced monosodium glutamate at different concentrations.EnglishAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)http://creativecommons.org/licenses/by-nc-sa/4.0/biostatisticslongitudinal and cross-section protocolmonosodium glutamateArticle2393-4603https://doi.org/10.61900/SPJVS.2025.04.16