Dănceanu Zara, Camelia-MihaelaPetrovici, AdrianaLăbușcă, LuminițaMinuti, Anca-EmanuelaStavilă, CristinaPlămădeală, PetruTiron, Crina-ElenaAniță, Dragoș-ConstantinAniță, Adriana-ElenaLupu, Nicoleta2025-12-292025-12-292024-10-21Danceanu-Zara, Camelia-Mihaela, Adriana Petrovici, Luminita Labusca, Anca Emanuela Minuti, Cristina Stavila, Petru Plamadeala, Crina Elena Tiron, Dragoş Aniţă, Adriana Aniţă, and Nicoleta Lupu. 2024. "Collection, Establishment and Assessment of Complex Human Osteocartilaginous Explants for Modeling Osteoarthritis" Biomedicines 12, no. 10: 2406. https://doi.org/10.3390/biomedicines12102406https://www.mdpi.com/2227-9059/12/10/2406https://repository.iuls.ro/handle/20.500.12811/6024With the increasing burden of osteoarthritis worldwide, cost efficient and reliable models are needed to enable the development of innovative therapies or therapeutic interventions. Ex vivo models have been identified as valuable modalities in translational research, bridging the gap between in vitro and in vivo models. Osteocartilaginous explants from Osteoarthritis (OA) patients offer an exquisite opportunity for studying OA progression and testing novel therapies. We describe the protocol for establishing human osteocartilaginous explants with or without co-culture of homologous synovial tissue. Furthermore, a detailed protocol for the assessment of explanted tissue in terms of protein content using Western blot and immunohistochemistry is provided. Commentaries regarding the technique of choice, possible variations and expected results are inserted.enCC BY 4.0https://creativecommons.org/licenses/by/4.0/osteoarthritissynovial tissueosteocartilaginous explantssample collectionex vivo modelsArticle2227-9059https://doi.org/10.3390/biomedicines12102406