Kosini, DanielNukenine, Elias N.Tchindebe, GeorgesTaimanga2026-06-082026-06-082026-05-282784-0379https://repository.iuls.ro/handle/20.500.12811/6542Soybean (Glycine max L.) is a key source of plant protein and oil in sub- Saharan Africa, but its storage is affected by the cowpea seed beetle, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae). The present study investigated the C. maculatus resistance of 10 soybean varieties in 2 agroecological zones of northern Cameroon and evaluated the protective efficacy of Gnidia kraussiana root extracts compared to neem seed oil on a susceptible variety. Significant varietal differences in C. maculatus susceptibility were observed, with resistance varying across locations. Only a subset of varieties (e.g., TGx1844-18E, SJ235, VT/SG Houla1, and Emgopa314) exhibited consistent resistance across both sites, and others showed location-dependent responses. Kodek/Houla1 displayed variable susceptibility and was classified as moderately susceptible in Maroua and moderately resistant in Ngaoundéré. Resistance was associated with seed biochemical traits. The soluble sugar content was positively correlated with susceptibility, whereas saponins were negatively correlated with susceptibility. Bioassays conducted on Kodek/Houla1 revealed that the hexane and acetone fractions of G. kraussiana root extracts caused high adult mortality, strongly reduced progeny emergence and seed damage, and prolonged protection during storage. At 5 g·kg-1, both fractions almost completely inhibited beetle development and showed an efficacy comparable to neem seed oil. These findings suggest that integrating soybean varieties with stable resistance profiles and plant-based protectants, such as G. kraussiana extracts, could contribute to the environmentally sustainable management of C. maculatus. However, further studies including chemical characterisation of the active compounds are required.EnglishAttribution 4.0 International (CC BY 4.0)http://creativecommons.org/licenses/by/4.0host biochemical defenceintegrated pest managementplant-derived insecticidespostharvest protectionresistance screeningArticle2784-0360https://doi.org/10.46909/alse-591205