Comparative boar and bull semen evaluation after percoll treatment

Abstract

There are numerous methods used in IVF both in bovine and pig for sperm separation and Percoll gradient is one of them. After using Percoll gradient spermatic parameters were as following: motility increased to 78.33% in boar samples and to 75% in bull samples; the number of live spermatozoa increased with 42.91%, 31.46% and 21.88% in boar sample, respectively with 19.37%, 9.34% and 5.85% in bull samples; normal spermatozoa increase to 98.52-98.92% in boar and to 84.22-93.97% in bull samples. Acrosome integrity, another parameter studied, indicate that in bull sample intact acrosome was before 83.97%, 87.58% and 88.1%, respectively 91.39%, 93.11% and 92.56% after using Percoll gradient, similar increase was in boar sample to 95.49%-98.42%. Therefore, Percoll is an easier and fast way to select viable and normal spermatozoa for IVF techniques