2026
Permanent URI for this collectionhttps://repository.iuls.ro/handle/20.500.12811/7278
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listelement.badge.dso-type Item , listelement.badge.access-status Open Access , Fractionation of transglutaminase produced by Streptomyces mobaraensis ATCC 27441(“Ion Ionescu de la Brad” University of Life Sciences, Iaşi, 2026) Strungariu, I.; Dumitrascu, M.; Nechita, E.; Stefan, M.Microbial transglutaminase (mTG) can catalyze covalent bonds between proteins, a property used in the food industry to restructure meat, improve the texture of dairy products, and extend the shelf life of foods. In our study, mTG was obtained using Streptomyces mobaraensis ATCC 27441 strain and fractionation using various ion exchange resins. The enzymatic activity was monitored using a spectrophotometric method. The results showed that the use of the Fractogel EMD SO3- resin led to the highest yield in the fractionation process (84.23%), recovering an enzymatic activity of 1613.83 U. Using Relisorb, a total enzymatic activity of 882.07 U and a yield of 46.04% was evidenced. In the case of Amberlite CG-50, a total activity of 869.558 U was achieved, with a final yield of 45.38%. The lowest final yield was recorded for Resindion SP 825L resin - 5.08%, with an enzymatic activity of 97.420 U. The results indicate that Fractogel EMD SO3 - is the most efficient resin for mTG fractionation, providing a robust approach for obtaining a high-purity enzyme suitable for industrial applications.listelement.badge.dso-type Item , listelement.badge.access-status Open Access , Assessment of dna purity and quantity in Teleorman Black Head, Suffolk and Ile de France sheep breeds(“Ion Ionescu de la Brad” University of Life Sciences, Iaşi, 2026) Ciobanu, A.; Ivancia, Mihaela; Pascal, Constantin; Simeanu, Daniel; Davidescu, Mădălina-Alexandra; Creangă, ȘteofilThe increasing demand for sheep meat requires modern methods to improve production, and genetic selection through genetic markers plays an important role in this process. The analysis of genetic markers depends on the quality of the extracted DNA, and assessing its purity through spectrophotometry is essential for the validity of genetic studies. In this study, blood samples were collected from three sheep breeds –Teleorman Black Head, Suffolk, and Ile de France. DNA was manually extracted using the Wizard Genomic DNA Purification kit (Promega), and purity was determined using the Nanodrop ASP-3700 spectrophotometer, ensuring precise evaluation of the A260/A280 ratio and DNA concentration. The DNA purity analysis of blood samples from the three sheep breeds demonstrates satisfactory results for the majority of samples. Out of 34 samples analyzed, 16 exhibited A260/A280 ratios within the ideal range of 1.7 to 2.0, indicating high-quality DNA suitable for further genetic analysis.. These results highlight the overall effectiveness of the DNA extraction process and support the reliability of subsequent genetic studies.