2016

Permanent URI for this collectionhttps://repository.iuls.ro/handle/20.500.12811/1234

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The morphopathological pravalence of inflammatory cardiomyopathies in dogs
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2015) Olariu Jurca, Adrian
The research was conducted during the period October 2013-May 2015 and it consisted of the necropsic exam of 30 dog bodies of different ages, sexes and breeds. It was completed with a microscopic exam of the histopathological preparations which were obtained from heart samples coming from 19 cases which presented lesions. Eight cases were diagnosed with inflammatory cardiomyopathies (42.09%). The diagnosed cardiac lesions, of the cases taken into study were multiple and complex, they developed in the same time or successively in several structures (pericardium, myocardium and endocardium as well as in blood vessels). They were exteriorized through pericarditis (5.26%), myocarditis (21.5%) and endocarditis (10.52%) as well as parasitic pulmonary arteritis (5.26%). Fibrinous pericarditis, chronic evolution, signaled in one of the cases was histopathologically exteriorized through the presence of fibrin networks with leukocytes and erythrocytes, attached to the swollen and infiltrated with inflammatory cells epicardium. The myocarditis which was morphopathologically identified, was represented by the parenchymatous myocarditis in one case, lymphohistiocitary myocarditis in two cases and fibrous interstitial myocarditis in one case. The endocarditis we diagnosed morphopathologically was represented by the verrucous or thrombotic endocarditis (5.26%) as well as by ulcero-polypous endocarditis (5.26%). In one case with cardiopulmonary dirofilariosis, we anatomopathologically diagnosed wormy pulmonary arteritis, expressed through lesions of several degrees, intensities and extensions of the arterial wall. They were translated through erosions of the intima as consequence of the contact with dirofilaria larvae.
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Xenobiochemic specificity of the deoxyribonucleic acid interaction with some cytostatic chemotherapeutics
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2015) Manolescu, Nicolae; Gârban, Zeno; Herman, Viorel; Sayti, Ludovic; Muselin, Florin; Baltă, Cornel; Marinescu, Sorin
The mostly recommended methods in oncotherapy are the surgical intervention, chemotherapy, radiotherapy, immunotherapy or a combination between them. The chemotherapy consists in the use of various drugs among which the most important are : the alkylating agents, antimetabolites, steroid hormones, antibiotics, phyto alkaloids, metal based drugs. In this review there are discussed the molecular mechanisms of the interaction of an alkylating cytostatic, i.e. cyclophosphamide (2 bis( -chloroethyl) amino-1-oxa-3-aza-2-phosphocyclohexan-2-oxide) – Cp and of a metal based cytostatic, i.e. cis-platinumum (cis-dichlorodiammineplatinum) - cDDP with deoxyribonucleic acid (DNA). Cyclophosphamide and cis-platinum present similar mechanism of interaction with DNA. Interacting with DNA these cytostatics give rise to mono- and bidentate adducts. The topics are of interest for comparative medicine (veterinary and human medicine). A particular importance arising from the appearance of the DNA-chemotherapeutic adducts is that these compounds can be detected analytically and can provide information on the consequences of the biochemical injury. Detection of DNA adducts is useful not only for the diagnosis of neoplastic diseases, but also for biomonitoring the evolution during chemotherapy.
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Researches on nutritive values on roe deer (capreolus capreolus) meat
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iasi, 2015) Lazăr, Mircea; Boișteanu, Paul-Corneliu; Lazăr, G.; Lazăr, Roxana
One of the species of cynegetic interest well represented numerically in the north of Moldavia, the roe deer (Capreolus capreolus) (Linnaeus, 1758) is a hunt species with high demand. Researches were developed on four roe deer: two males and two females, harvested in the north area of Moldavia (counties Suceava and Iaşi). The assessment of meat quality represents a process which includes the analyses of physico-chemical and organoleptic parameters, the establishment of nutritional, biological, food and culinary values. Samples were processed in order to determine proteins collagen, fat, water, and the pH, known as that the nutritional value of meat is given by these parameters and expression of the sensorial characteristics based on relationships in which they are found. The obtained results reflect the high nutritional value of meat from deer, and from an alimentary point of view the meat came from females situates in consumers top preferences.
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Determination of biofilm production in animals originated pseudomonas aeruginosa strains
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Ciocan Moțco, Oana-Alexandra; Çiftçi, Alper; Carp Cărare, Mihai; Mareș, Mihai; Guguianu, Eleonora; Rîmbu, Cristina-Mihaela; Grecu, Mariana; Carp Cărare, Cătălin
For the detection of Biofilm formation method, total 56 clinical isolates viz. Pseudomonas aeruginosa were used. Strains were identified using standards microbiological procedure. The susceptibility test of biofilm producing bacteria was performed by using disc diffusion technique. Biofilm detection was tested with Congo Red Agar method (CRA). These method is rapid, sensitive and reproducible, this method is suitable for detection of biofilm formation in the present study. Out of 56 isolates, CRA method detected 17 (30,35%) as high biofilm producer, and 39 (69,65 %) biofilm non- producer. According to the antibiotic susceptibility test, higher antibiotic resistance was observed in biofilm producing bacteria than non-biofilm producers.
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Epidemiological study of the respiratory infectious pathology in goats in north-estern romanian region
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Mihai, Iuliana; Velescu, Elena; Tanase, Irina-Oana; Rusu, Oana-Raluca
Abstract The national and European small ruminant increasing number during the 2010-2014 period is the reason for starting studies and researching about specific pathology. The purpose of this study is to establish a local statistic concerning the prevalence of the respiratory infectious pathology for the goats from North-Eastern Romanian region, in the counties: Iasi, Botosani and Vaslui on a number of 4608 goats during the period 2014-2016. Since respiratory diseases are the most frequent on the goats, most knowledge are obtained from cattle and sheep pneumonia analogies or from personal experience. Clinical manifestation are caused by following viral agents: Parainfluentza 3 virus, Respiratory Syncitial virus, Maedi Visna virus, Caprine Arthrithis Encephalitis virus, compounded by secondary complication caused by Manheimia haemolytica and Pasteurella multocida. Research has led to establishment of the prevalence of respiratory disease in goats counties investigated during the study period. The most affected breeds are imported breeds or their half-breeds: Saanen 27% and French Alpin 34%, kids are more sensitive (60%) compared with adult animals (38%), with 44.4% of the morbidity and mortality rate 12%. Knowing the epidemiological situation facilitates drawing up plans to combat specific prevention and protection that allow herds of goats.
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Serological investigations on Aujeszky’s disease virus in farm pigs from Iași County
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Aniță, Adriana-Elena; Aniță, Dragoș-Constantin; Savuța, Gheorghe; Ludu Oșlobanu, Luanda-Elena
Aujeszky's disease caused by Aujeszky's disease virus (ADV) is one of the most important diseases in swine industry. The infection is almost asymptomatic in older pigs and in adults a latent infection persists lifelong. In this study, we conducted a serological survey of ADV in farm pigs in Iași County. In total, 172 pig serums collected between August and September 2016 were screened for the presence of antibodies against ADV. For the screening was used the ELISA kit Svanovir®PRV-gE-Ab, that can differentiate the vaccinated pigs from the infected ones based on the detection of antibodies against the gE antigen. In all four investigated farms positive animals for ADV antibodies were detected, the overall prevalence registered being 36,62%. Our study is a preliminary investigation underlining the possibility of ADV circulation and persistence in swine farms from Iași County.
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Metabolical changes in HBV/ HIV coinfections
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Mărculescu, Afrodita-Doina; Bejan, Codrina; Mareci, Daniel; Trincă, Lucia-Carmen; Gavăt, Cristian-Cătălin
The aim of this study was to evaluate the main metabolic changes in the case of hepatitis B virus (HBV) / human imunodeficiency virus (HIV) coinfection. A retrospective study, on 482 HIV infected patients was assesed, at Iasi Regional HIV-AIDS Center Iasi, between 2000-2014. Subjects were divided into 2 groups, according to the presence or absence of HBV coinfection. HIV prevalence was higher in the 20-29 years aged group (86.5%), parenteral routes being the predominant mode of HIV transmission (61.5% vs 58.5%). Mean ALT levels were significantly higher (p<0.001) in the HBV group (49.92 IU/L vs 32.93 IU/L). Average total cholesterol levels were significantly higher (p<0.001) in the HBV group( 182.58 vs 167.59 mg%). The average levels of serum triglycerides in the HBV group were significantly lower (p<0.001) than those recorded in the nonHBV group (130.72 vs. 164.59 mg%). Dyslipidemia was common in the HIV/HBV coinfected group (107 vs. 82). Hepatitis B virus infection induced a 2-fold higher relative risk for the occurence of hepatic cytolysis syndrome .
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Phisiotherapy efficiency in dogs with polyradiculoneuritis
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Ursache, Alexandru; Henea, Mădălina-Elena; Tipa, Georgiana-Andreea; Stanciu, Gabriela-Dumitrița; Solcan, Gheorghe
Idiopathic polyradiculoneuritisis an acute demyelinating disease characterized by inflammatory damage of the entire peripheral nervous system. In veterinary medicine it is more common in dogs and rarely diagnosed in cats. This rare disease is characterized clinically by progressive flaccid quadriplegia and hyporeflexibility, facial paralysis and laryngeal weakness. The aim of the current study was to describe the physiotherapy treatment methods for patients diagnosed with this condition. Recovery after physiotherapy sessions in patients diagnosed with idiopathic polyradiculoneuritis was reported in 5/6 cases. Physical therapy is the main method of recovery, requiring close collaboration between veterinarian and owner for positive results.
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Ameliorative effect of cinnamon aqueous extract against hypercholesterolemia induced hepatic alterations in rats
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Farouk Mahmoud, Mona
This study was done on four groups of male wistar rats. Control group, cinnamon aqueous group which fed on 200 mg/kg/day cinnamon aqueous extract orally, control positive group which was fed on a high cholesterol diet and treated group and was fed a high cholesterol diet and given cinnamon aqueous extract orally at 200 mg/kg/day for two months to evaluate the ameliorative effect of cinnamon aqueous extract on the alterations in liver induced by hypercholesterolemia. The histopathological findings showed vacuolar degeneration in some areas of the hepatic tissues. Diffuse fatty degeneration was noticed in most of the hepatocytes. Thickening in the CT surround the central and portal vein. The hepatic cells showed faint positive immunostaining reaction forKi67. In the treated group regeneration was noticed in most of the hepatocytes. The cells showed cytoplasmic degranulation especially the cells in the triad areas. Proliferation of the vonkupher cells was noticed. The hepatocytes showed positive immunostaining for Ki67 which indicate cell viability.Also theaddition of cinnamon aqueous extract adjusts the highest level of cholesterol, triglycerides, and low-density lipoprotein.
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Protocol developing for identification of vegetal matrices used in ammodytes ammodytes freeze-dried venom adulteration
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Tulcan, Camelia; Ahmadi, Mirela; Huțu, Ioan; Milovanov, Cornelia; Radulov, Isidora; Alexa, Ersilia; Obistioiu, Diana; Folescu, M.; Mircu, Călin; Boldura, Oana-Maria
Presence of corn flour adulteration was detected by extracting the DNA from 25mg of freeze dried venom and using it as template in PCR amplification with zein specific primers known to be highly specific for corn species. The obtained amplicon was purified from agarose gel and sequenced in order to further confirm the presence of corn specific DNA sequences. The sequence thus obtained was uploaded in a DNA Data Base, and aligned with the reference zein sequence. The 99% of similarity between the two sequences enables us to confirm the corn flour adulteration in the analyzed venom sample.
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Morphological features of the axial skeleton to badger (Meles Meles)
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Spătaru, Constantin
Studies on the axial skeleton in badger reveal the specific morphological features such as: the tlas looks like the same in carnivores having the alar hole united with its vertebrae hole through a duct alar and the transverse hole is oppened on the caudal edge of wings. The cranial articular surfaces are concave dorso-ventrally spaced between them allowing the flexion and extension. The odontoid process looks cylindrical having the cranial jointing surfaces slightly convex which continue the articular surface which are present on the ventral side of the odontoid process. The cervical vertebrae are large with the wide interarcuale spaces like in pigs, something that allows hyperextension of the neck and with the lamellar aspect of the transverse processes. The thoracic vertebra present the costotransversale processes detached from the vertebral body, with slightly concave surfaces costotransversale joint at the first half of vertebrae and flat to the last half. Mamilo-articular processes exceed the half of the spinous processes height and the jointing surface being slightly concave. The sacrum shows the median sacral crest with three spaced spine with cranio-caudal decrease, flanked by the symmetrical suprasacrale spine which has two holes that open both dorsally and ventrally. Examining the aspects of the axial skeleton is remarkable the morphological similarity of these structures with those of pigs and canids.
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The morphological peculiarities of budger skull (Meles Meles)
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Spătaru, Constantin
The studies were performed on badger skulls prepared by removal of adjacent tissues for evidence of morphological peculiarities such as: the shape, length and development processes of the bone and specific joints. The appearance of the bony prominences demonstrates both the traction force in contraction and pressure produced by muscles on the bones, in accord with the anatomical principles. Analyzing these issues, at badger' skull ones can observe the canines which are developed, having strong root noticed by extended alveolar canine which protrude on the surface of maxillary bone. The incomplete orbital cavity is laterally delimited by the zygomatic process of the frontal and the frontal process of zygomatic bone, both being reduced and triangular. Caudo-medial (dorsal and ventral) the orbit is bounded by each high blade bone which converge toward the orbital hiatus. The ceiling of mouth looks trapezoid and narrows caudal because the layout of the last molar cross-like carnivores. The articular condyle of the temporal appears as a groove, transversely elongated, caudal flanked by the retroarticular process. The mandible has branches diverged caudally, causing widwning of oropharyngeal opening and articular condyle of the mandible looks cylindrical and transverse elongated. The developed coronoid process is quadrangular and the angular process is very developed.
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Comparative morphological peculiarities of the skulls at some rodents (rabbit, nutria, guinea pig and squirrel)
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Spătaru, Mihaela-Claudia
Generally, the rodent skulls have a rectangular shape, viscerocranium being about 2/3 of its length, representing the dominant portion of the skull. As a result of studies performed on the skulls from rabbit, guinea pigs, squirrels and nutria, several differences are observed in the matter of skulls morphology which is determined primarily by grasping and trituration of the food, even though these animals are rodents. At all rodents, the incisive bones are dominant, having a strongly rostral curved aspect. The convexity continues with the incisors, in their turn highly curved and strong. In rabbits, the jointing condyle is highly exalted above the mandible molar level which reduces the sectioning force through vertical action and its jointing surface is transversally orientated, allowing the flexion, extension or the propulsion and the caudal movements, like all herbivores. Articular condyle in nutria exceeds about 1 cm the surface of the mandible molars plane, this species requires an increased force for severing food, both in the terrestrial or aquatic environment. In guinea pigs, a terrestrial species, the condyle jointing surface is located near the molar level easing the food trituration by vertical and lateral movement. At squirrel, the condyle is placed nearly in the mandible molars plane but the ascension of the coronoid process above the molars plan eases the antero-caudal movements more than the vertical sectioning.
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Comparative morphological peculiarities of the hindlimb to some rodents (rabbit, nutria, guinea pig and squirrel)
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Spătaru, Mihaela-Claudia
Although all the species proposed to study are rodents, that four mammal live in different environments (terrestrial, semi-aquatic or semiarboricol) and develop survival strategy involves in locomotor adaptation to the needs imposed by the environment. Thus, the rabbit develops a terrestrial moving by leaps and bounce, the length of the hind limb is double compared to the frontlimbs, muscles propellant are highly developed, through action on ischial insertion lead to appear a three massive ischial tubers in other rodent species instead marked by a ridge or a rounded small tuber. On the other hand, a strong flexors of the hip (mainly muscles as superficial, middle and accessory gluteus) lead to lengthening the iliac blade to nutria, squirrel and guinea pigs and the emergence of deep gluteal pits separated by a high ridge. At nutria and squirrel, the femur is seconded by a long neck and has ¾ articular surface of a sphere and to rabbit and squirrel, very short in guinea pig , is observed the presence of the third trochanter.
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Observations on the sexual behavior of the tarantulas from the species A. Geniculata
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Gașpar, Corneliu; Grămadă, Iulian-Cătălin; Ailincăi, Luminița-Iuliana
The sexual behavior of tarantulas is very differently from that of higher vertebrates due to their eco- morphological and physiological features. The male, who has a pair of sex bulbs, in the presence of a receptive female will perform a "dance" that produces vibrations, to which the female respond depending on the willingness to mating.
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Observations on the maternal behavior of Madagascar cockroaches (Gromphadorhina portentosa)
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Gașpar, Corneliu; Grămadă, Iulian-Cătălin; Ailincăi, Luminița-Iuliana
This paper aims is to observe the nesting behavior of Madagascar cockroaches. Although, after laying eggs, the implication of parents insect is zero or minimal, however, it was observed in this ovoviviparity species a feature absolutely unique that the female takes care of nymphs after hatching of 60 days, behavior that may be considered maternal .
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Staphylococcus aureus isolated from intramammary infection in immunologically protected goats
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Rîmbu, Cristina-Mihaela; Guguianu, Eleonora; Carp Cărare, Cătălin; Horhogea, Cristina-Elena; Tanase, Irina-Oana; Crețu, Carmen; Rusu, Oana-Raluca; Grecu, Mariana; Todose, Ana; Golban, Rita
In a goats farm with 86 goats immunologically protected by vaccination against gangrenous mastitis, evolved clinical and subclinical intramammary infections. 30 milk samples from goats with clinical mastitis evolving and 16 milk samples from apparently healthy goats were collected. Following bacteriological exams 36 Staphylococcus aureus strains were isolated and identified using standard protocols, 25 (69.44%) being isolated in pure culture and 11 (30,55%) in association with other bacteria: Escherichia coli, Pasteurella haemolytica, Klebsiella oxytoca. Bacterial strains were morphologically identified using Gram staining and biochemical tests. The antibiotic susceptibility was tested for 15 strains of Staphylococcus aureus on 10 antibiotics and showed similar patterns.
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The comparative evaluation of antibiotic therapy in the treatment efficiency of chronic bronchitis in horses
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Nadăș, George-Cosmin; Chirilă, Flore; Bouari, Cosmina-Maria; Fiț, Nicodim-Iosif
The susceptibility testing of microbial pathogens obtained by transtracheal lavage and bronchoalveolar lavage from the respiratory system of horses and cultured were compared. The antibiotics were compared with each other (inhibition zone diameter and resistance/sensibility of etiologic agents) to determine the best antibiotic that can be administered in horses with respiratory infections. Comparison of different families of antibiotics were also done (by average inhibition area diameter). Among the samples obtained by means of a bronchoalveolar lavage there were no microorganisms with resistance to florfenicol, enrofloxacin, and amoxicillin. The highest average inhibition zone diameters were: penicillin (36 mm), ciprofloxacine (25 mm), enrofloxacin (22.5 mm) and tetracycline (20 mm). The best antibiotics for treating the pathogens in samples obtained by means of a transtracheal lavage were: cefquinome (23 mm), tetracycline (21 mm), trimetoprim (21 mm) and florfenicol (19.83 mm). Further (and more elaborate) studies are required to determine the best antibiotics for the treatment of respiratory infections.
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Identification and antifungal susceptibility testing of some candida Spp. strains using modern phenotypic methods
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Nadăș, George-Cosmin; Chirilă, Flore; Bouari, Cosmina-Maria; Fiț, Nicodim-Iosif
The aim of this study was to identify Candida isolates from animals and humans using VITEK 2 card system, a modern phenotypic method testing 64 biochemical characters and the susceptibility testing using MIC method on the same Vitek 2, for 6 modern antifungals. The research was conducted during January-June 2016 in the Department of Microbiology, Faculty of Veterinary Medicine in Cluj-Napoca. Identification was made from 48 hrs isolated colonies, preparing a suspension of 1.8-2.2 optical density, and 280μl were substracted and transferred over in saline for susceptibility testing. After a period of 18-19 hours for analysis of 64 biochemical characters for each tested strain, the results were saved/printed as Laboratory report in PDF format. From the total of 20 tested strains, six were represented by Candida albicans, five strains were identified as Candida krusei, 3 were Candida tropicalis 2 Candida catenulata 2 Candida parapsilosis and other two Cryptococcus laurentii. Most identified species are susceptible to micafungin with MIC ranging from 0.06 and 0.12 depending on the species, except for Candida parapsilosis. Voriconazole was also very efficient, with MIC value of 0.12 for almost all identified species. Caspofungin efficiency is specie-related, so for C. albicans and C. tropicalis MIC is 0.25, for C. krusei is 0.5 and for C. catenulata and C. parapsilosis MIC equals 1. Flucytosine has a constant value of 1 in all identified species, except for C. krusei with MIC value of 4 or 8 - resistant strains.
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Using contrast computed tomography (CT) in the diagnosis of some canine male genitalia disorders
(”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2016) Irimie, Alexandra; Lăcătuș, Radu; Groza, Ioan-Ștefan; Pop, Alexandru-Raul
The ultrasound examination is a powerful tool in diagnosing canine male genital disorders, but presents some shortcomings in the exact localization of some intraabdominal masses and their vascularization. Some disorders, such as abdominal sertolinomas, atrophic testicle, persistent Müllerian ducts (PMDS) or testicle cord torsions, can be difficult to diagnose and pinpoint if the obvious symptoms (for example feminization, alopecia and skin hyperpigmentation) are not present. The purpose of this study was to test the accuracy of contrast computed tomography (CT) techniques compared to the ultrasound exam (US), in diagnosing some male reproductive disorders. The study was conducted on 7 male canine patients, pure breed, with the age between 3 months and 7 years old. After the patients were presented at the clinic an ultrasound in B-Mode and Doppler was performed using a Mindray DC3 Vet equipment. The CT contrast agent was Visipaque (iodixanol, 320 mgI/mL, producer Nycomed Amersham) using automated injection after the patient was general anesthetized, the dose being automated adjusted by the device. The results are inconclusive; the retained testicle ca be easily observed but the uterine artery as well as the reminiscent uterine horns are too small to visualize. The disadvantage of the CT is besides the radiation; the patient needs to be under general anesthesia. In conclusion, in some cases, such as cryptordism, sertolinomas, lymphnode metastasis or intraabdminal masses, the CT is very efficient in pinpointing the exact location, but in other cases such as PMDS the contrast CT is not recommended, but the US is.

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