2024

Permanent URI for this collectionhttps://repository.iuls.ro/handle/20.500.12811/4533

Browse

Now showing 1 - 20 of 25

listelement.badge.access-status Open Access ,
Non-thermal plasma (NTP) treatment of Trigonella foenum-graecum L. seeds stimulates the sprout growth and the production of nutraceutical compound
(BioMed Central, 2024-01-06) Motrescu, Iuliana; Lungoci, Constantin; Ciolan, Alexandru; Jităreanu, Gerard
The possibility to stimulate the production of some nutraceutical properties of fenugreek (Trigonella foenum-graecum L.) sprouts by non-thermal plasma (NTP) processing of the seeds in different conditions was studied. The non-thermal plasma used in this work was a surface dielectric barrier discharge. Two types of processing were performed: direct NTP treatment and NTP with a cover treatment, to simulate the processing of packaged seeds. For all treatments, the effect of pre-soaking of the seeds was studied as well. The analyses of the seeds after processing indicated an increase of the hydrophilicity of their surface for NTP direct treatment as resulted from the water contact angle measurements, which could be due to the strong etching evidenced by scanning electron microscopy imaging. A significant (p < 0.05) increase of the seedling growth, by up to 50%, was found especially for the pre-soaked seeds. These results were correlated with the increase of chlorophyll pigments concentrations, with higher concentrations in the case of NTP direct treatment than for the NTP with cover treatments. Direct NTP treatment for 30 s of dry seeds led to the highest increase of the flavonoid concentration of about three times compared to that obtained for untreated seeds. For the polyphenols and antioxidant activity, NTP with cover treatments proved to be better, with a significant increase, especially for 90 s treatment of the pre-soaked seeds. All the results indicate the possibility of tuning the nutraceutical properties of fenugreek sprouts by NTP treatment.
listelement.badge.access-status Open Access ,
Experimental Study on Rats with Critical-Size Bone Defects Comparing Effects of Autologous Bone Graft, Equine Bone Substitute Bio-Gen® Alone or in Association with Platelet-Rich Fibrin (PRF)
(MDPI, 2024-05-24) Ciobanu, Petru; Danciu, Mihai; Pascu, Andrei; Gardikiotis, Ioannis; Forna, Norin; Sîrbu, Mihnea-Theodor; Calistru, Anca-Elena; Puha, Bogdan; Veliceasa, Bogdan; Sîrbu, Paul-Dan
Background: A critical-sized bone defect (CsBD) is considered one that will not heal spontaneously and requires reconstruction. This study aims to compare the results of using different bone reconstructive techniques and to study the potential of platelet-rich fibrin (PRF) to enhance the healing properties of a bone substitute (BS). Methods: In this experimental study on rats, the treatment of critical-sized bone defects was carried out by analysing four groups: a control group in which the bone defect was left empty; a group treated with Bio-Gen®; another group in which the defect was treated with PRF in combination with Bio-Gen®; and the last that was treated with autologous bone graft (ABG). The defects were evaluated by microcomputed tomography (µCT) and then histomorphometrically. Results: From both the histological and imagistic point of view, the best results were registered in the ABG group, followed by the group treated with Bio-Gen® with PRF, Bio-Gen® group, and control group, with statistically significant differences. Conclusions: A 5 mm defect in the rat radius can be considered critical. ABG showed the best results in treating the bone defect. PRF significantly enhanced the efficacy of Bio-Gen®.
listelement.badge.access-status Open Access ,
Aging of Superficial Musculoaponeurotic System of the Face-Novel Biomarkers and Micro-CT Relevance of Facial Anti-Gravity Support
(MDPI, 2024-05-24) Hînganu, Marius-Valeriu; Cucu, Ramona-Paula; Costan, Victor-Vlad; Lozneanu, Ludmila; Tamaș, Camelia; Calistru, Anca-Elena; Hristian, Liliana; Hînganu, Delia
The soft superficial tissues of the face are against gravity through an intricate network of ligaments and ligamentous attachments. The aim of this investigation is to delineate the relationship between the muscular, fibrous, and vascular components of the superficial musculoaponeurotic system of the face (SMAS) at the level of its periosteal fixation areas from advanced radiological and novel biomarkers’ perspectives. These areas represent key points underlying skin aging and the longevity of restorative surgery results. Methods: This study was carried out on 37 surgical specimens, excised from patients admitted for surgery. On the excised specimens, we used special immunohistochemical techniques, such as markers for collagen type III, angiogenesis, vascular endothelium (I-CAM2) and muscle fibers (MYH2). We performed a micro-CT evaluation of these 37 specimens. Results: The results of this study showed different radiologic and IHC characteristics of the means of periosteal fixation of the SMAS. Evidence of morphohistological and radiological peculiarities of the retaining ligaments highlights new data for future functional studies of these structures. Our research must be continued with larger groups of subjects and through detailed methodological studies of vascular microperfusion and could represent an important new step in biotissue engineering and the customization of surgical techniques involving the sub-SMAS layers.
listelement.badge.access-status Open Access ,
High Energy Pulsed Laser Beam to Produce a Thin Layer of Crystalline Silver without Heating the Deposition Substrate and Its Catalytic Effects
(MDPI, 2024-06-19) Cocean, Alexandru; Cocean, Georgiana; Postolachi, Cristina; Garofalide, Silvia; Pricop, Daniela-Angelica; Munteanu, Bogdănel-Silvestru; Bulai, Georgiana; Cimpoeșu, Nicanor; Motrescu, Iuliana; Pelin, Vasile; Ababei, Răzvan-Vasile; Dimitriu, Dan-Gheorghe; Cocean, Iuliana; Gurlui, Silviu
Crystalline silver thin layers were obtained using high-energy pulsed laser ablation without the heating of the deposition substrate. The fluid Plateau–Rayleigh (PRI), Rayleigh–Taylor (RTI), and Richtmyer–Meshkov (RMI) instabilities, as well as the crown splash induced during the pulsed laser deposition (PLD) in the high energy regime, resulting in ring and pearl-shaped structures, offer the benefit of an increased sorption surface. These morphological structures obtained for the silver thin layers make them of interest for catalytic applications. This study addresses both fundamental and applied issues on the morphological structures obtained for the silver thin layers and their catalytic function in organic processes. In this sense, the catalytic action of the thin silver layer was highlighted by modifications of the Reactive Blue 21 dye (C.I.) in an aqueous solution with sodium bicarbonate. Specific investigations and analyses were carried out using electron microscopy and elemental analysis (SEM-EDX), atomic force microscopy (AFM) and profilometry, mass spectrometry, ablation plasma diagnosis, diffractograms (XRD), as well as IR spectroscopy (FTIR). In addition to the experimental investigation and analyses, the simulation of the ionization energy threshold was conducted in COMSOL for complementary evaluation on the involved processes and phenomena.
listelement.badge.access-status Open Access ,
Briquette Production from Vineyard Winter Pruning Using Two Different Approaches
(MDPI, 2024-07-09) Țenu, Ioan; Roșca, Radu; Corduneanu, Oana-Raluca; Roman, Cecilia; Șenilă, Lăcrimioara; Arsenoaia, Vlad-Nicolae; Butnaru, Liviu; Băetu, Marius; Chirilă, Constantin; Cârlescu, Petru-Marian
Worldwide, different strategies are being developed in order to ensure optimum conditions for the development and growth of economic competitiveness, as well as for increasing the quality of life and environmental protection. All these strategies are closely linked to the development and modernization of systems for producing energy from clean and renewable sources. In this context, the present paper presents the results of research regarding the evaluation of the sustainability of briquette production using biomass resulting from vine winter pruning as the raw material. An analysis of the scientific literature indicates that nearly 8 Mt of biomass would result from the over 7.4 million hectares of vine plantations in the world, biomass that could be valorized through densification in order to produce solid biofuels with a lower calorific value of more than 17 MJ/kg. This study examines the production of briquettes from vineyard winter pruning with consideration of two types of densification technologies: baling and natural drying of the tendrils, and collection, shredding, and artificial drying of the lignocellulose debris. The quality indices and energy consumption and energy efficiency of the briquettes were evaluated to determine their feasibility as an alternative fuel source. When designing the scientific endeavor, the following aspects were considered: defining the aim and objectives of the research; designing the research algorithm; collecting, preparing, and conditioning the biomass; conducting a chemical analysis of the briquettes; and evaluating the energy consumption and energy efficiency for producing the briquettes, taking into account two drying methods (natural and artificial drying). In the meantime, some specific laboratory equipment was designed and built for the artificial drying of biomass, evaluation of mechanical durability, measurement of energy consumption, etc. Analysis of the experimental data has led to the conclusion that the agricultural waste from vine pruning can constitute an important and sustainable source of energy in the form of briquettes that fulfill most of the requirements imposed by international standards.
listelement.badge.access-status Open Access ,
Evaluation of the Functional Parameters for a Single-Row Seedling Transplanter Prototype
(MDPI, 2024-02-24) Vlahidis, Virgil; Roșca, Radu; Cârlescu, Petru-Marian
The development of an automatic seedling planting system for micro-farms requires testing under laboratory conditions to verify the theoretical relationships between essential functional parameters, working speed and planting time. The constructive dimensional values of the prototype, the results measured in stationary mode directly on the transplanter and the auxiliary equipment and the direct determinations of the working parameters on the soil bin are used. Depending on the characteristics of the soil bin trolley, a range of speeds is chosen at which the machine is tested. The data obtained validate the correct operation of the prototype at speeds close to those determined theoretically for the following indicators: distance between plants per row, planter wheel slippage, misplanted seedlings rate and seedling frequency, with results comparable to existing agronomic standards. Once the appropriate operating speeds of the machine have been obtained, between 0.304 and 0.412 m/s, with planting frequencies between 0.899 and 1.157 s−1 (respectively, 53.94 and 69.42 seedlings per minute), optimizations and adjustments of some machine components can be made, for subsequent testing in real field conditions.
listelement.badge.access-status Open Access ,
Tillage and Straw Management Practices Influences Soil Nutrient Distribution: A Case Study from North-Eastern Romania
(MDPI, 2024-05-07) Calistru, Anca-Elena; Filipov, Feodor; Coroi Cara, Irina-Gabriela; Cioboată, Marius; Țopa, Denis-Constantin; Jităreanu, Gerard
Tillage practices govern crop quality and quantity through soil nutrient availability and crop root systems. A deeper knowledge of the impact of conservation tillage on soil chemical characteristics (such as pH, soil organic carbon, macro and micronutrient storage and distribution) is required for both the promotion of agricultural sustainability and environmental preservation. This study assesses the changes in soil features and properties in the context of a long-field experiment with different tillage systems and straw management practices. Research findings revealed that compared with conventional tillage (CT) conservative tillage with partial straw retention (MT) and no-tillage with straw mulching (NT) substantially boosted the organic carbon (OC) (by 6–19%), total nitrogen (TN) (by 2–12%), and available potassium content (AK) (by 2–5%), in 0–30 cm soil depth. However, the stratification trend was observed for available macro and micronutrient content (Zn, Fe, Mn) in both conservative management practices. The concentration of Cu indicates a constant pattern through a 0–30 cm soil profile with a higher concentration under MT (1.41 mg kg−1) compared to NT (1.10 mg kg−1). In particular, the results failed to establish if conservation tillage can increase the total phosphorus (TP) and potassium content (TK), where only in surface 0–10 cm an increase was observed. This research also suggested that the X-ray fluorescence analysis (XRF) of total micronutrient content (Zn, Cu, Fe, Mn) is minimal or unpredictable with no substantial differences between the tillage systems and straw return management practices. These findings suggest that conservation tillage in north-eastern Romania might be optimal to maintain soil quality status and sustain high yields.
listelement.badge.access-status Open Access ,
Insights into the environmental benefits of using apple pomace for biosorption of lead from contaminated water
(Elsevier, 2024-08-23) Ungureanu, Gabriela; Enache, Iuliana-Maria; Coroi Cara, Irina-Gabriela; Motrescu, Iuliana; Patraș, Antoanela
The apple processing industry generates large quantities of organic waste, presenting a major source of organic contamination. Consequently, finding an effective solution for valorizing this waste has become a pressing issue. This study aims to address two key concerns: (i) solving an agricultural problem by efficiently using agri-food residue, and (ii) removing lead, an extremely toxic element, from contaminated waters to mitigate environmental pollution. Two biosorbents were tested: raw apple waste (RA), obtained from a mixture of apple varieties, and the same material after extracting valuable bioactive and reusable components, extracted apple (EA). The study evaluated the influence of pH, initial biosorbent mass, adsorption kinetics, and equilibrium isotherms. The results are very promising, showing a lead removal efficiency of 82 % for RA and 100 % for EA at a low initial concentration of the solution of 20 mg Pb2⁺/L and an optimal pH of 5 ± 0.5. The Langmuir model predicted a maximum adsorption capacity of 44.6 mg/g for RA and 48.6 mg/g for EA. These findings demonstrate that apple waste, even after selective extraction of valuable bioactive components, can be effectively used for environmental remediation on a practical scale.
listelement.badge.access-status Open Access ,
A Study on the Development of Two Ornamental Varieties of Ipomoea batatas Cultivated in Vertical Systems in the Northeastern Region of Europe
(MDPI, 2024-01-30) Cojocariu, Mirela; Marta, Alina-Elena; Jităreanu, Carmenica-Doina; Chelariu, Elena-Liliana; Căpșună, Sorin; Coroi Cara, Irina-Gabriela; Amișculesei, Petronica; Hangan Istrate, Ana-Maria-Roxana; Chiruță, Ciprian
The Ipomoea batatas ornamental variety of the edible sweet potato is a decorative sweet potato with exquisite leaves, available in different varieties. Due to its numerous characteristics related to resistance, adaptability to environmental conditions, rapid growth, and aesthetic traits, it has attracted the interest of researchers. The purpose of this study is to investigate the growth and development behaviour of two varieties of I. batatas, namely ‘Margarita’ and ‘Blackie’, cultivated in vertical systems, to provide guidelines for their usage in green façade design. The physiological growth processes throughout the growing season were analysed by monitoring three biometric parameters (diameter, height, and leaf count), transpiration (dehydration rate), and the photosynthesis process (photosynthetic pigment content) for treated and control variants and four cardinal orientations (N, E, S, and W). Compared to the control and western orientation, which proved to be less advantageous, the northern orientation and treatment appeared to be the most suitable options for both varieties analysed. Also, the content of organic substances was more intense in the Cropmax-treated ‘Blackie’, especially on the northern façade, with the highest quantities of chlorophyll a (17.1%), chlorophyll b (9.1%), and carotenoid pigments (7.5%). In addition, the leaf dehydration rate, as well as the amount of free water loss, were reduced on the northern façade of ‘Blackie’. Therefore, ‘Blackie’ can be recommended as a variety distinguished by its improved resistance to challenging environmental conditions, particularly drought.
listelement.badge.access-status Open Access ,
Adsorption of Ni(II) from Aqueous Media on Biodegradable Natural Polymers—Sarkanda Grass Lignin
(MDPI, 2024-04-18) Ungureanu, Elena; Samuil, Costel; Țopa, Denis-Constantin; Ungureanu, Ovidiu C.; Tofănică, Bogdan-Marian; Fortună, Maria-Emiliana; Brezuleanu, Carmen-Olguța
Heavy metals are pollutants that pose a risk to living systems due to their high toxicity and ability to accumulate and contaminate. This study proposes an alternative approach to the static adsorption of Ni(II) from aqueous media using Sarkanda grass lignin crystals, the non-cellulosic aromatic component of biomass, as an adsorbent substrate. To determine the best experimental conditions, we conducted tests on several parameters, including the initial and adsorbent solution pH, the concentration of Ni(II) in the aqueous solution, the amount of adsorbent used, and the contact time at the interface. The lignin’s adsorption capacity was evaluated using the Freundlich and Langmuir models to establish equilibrium conditions. The Lagergren I and Ho–McKay II kinetic models were used to determine the adsorption mechanism based on surface analyses and biological parameters such as the number of germinated seeds, energy, and germination capacity in wheat caryopses (variety Glosa) incorporated in the contaminated lignin and in the filtrates resulting from phase separation. The results suggest that Sarkanda grass lignin is effective in adsorbing Ni(II) from aqueous media, particularly in terms of adsorbent/adsorbate dosage and interfacial contact time.
listelement.badge.access-status Open Access ,
Quality Parameters of Plum Orchard Subjected to Conventional and Ecological Management Systems in Temperate Production Area
(MDPI, 2024-08-27) Rusu, Mariana; Coroi Cara, Irina-Gabriela; Stoica, Florina; Țopa, Denis-Constantin; Jităreanu, Gerard
Environmental protection, global food security, and nutritional quality are critical issues for worldwide sustainable development. Plums (Prunus domestica L.), well-known for their rich nutritional content and distinct phytochemical profile, have received increased attention due to their potential health benefits. The present study evaluates the ecological and conventional management systems of quality parameters in three plum varieties—Tuleu Gras, Record, and Centenar—and establishes suitable practices to improve fruit quality and yields. The fruit morphometric features (color, firmness, soluble solid content, titratable acidity, and total sugar) were analyzed during their raw fruit state, while different fruit-sample extracts were assessed for phytochemical compounds and heavy metal concentrations, specifically, zinc, copper, nickel, and cadmium. The results show a wide variability in the examined characteristics among management practices that differentially accumulated throughout the ripening phase and significantly influenced the nutritional value of the plum fruit. The application of an ecological management practice yielded small plum fruits (38 g) and maintained a more consistent and vigorous fruit color compared with the conventional system (83 g). Moreover, ecological plums have higher antioxidant activities, total polyphenols, and flavonoids (21.70–25.54 µM Trolox/g of dw, 3.89–7.76 mg GAE/ g of dw, and 1.45–3.65 mg CE/g of dw, respectively). Among the content of metals in the plum fruit, copper exhibited the highest concentrations (0.83–1.21 mg/kg), while cadmium was detected at the lowest levels (0.006–0.009 mg/kg). The potential health implications of heavy metals, by calculating their estimated daily intake (EDI), target hazard quotient (THQ), and hazard index (HI), for both adults and children were also analyzed. The EDI values of accumulated elements in the plum fruits followed the order of Cu > Zn > Ni > Cd with no concern for human health (THQ and HI < 1). According to this study’s findings, fruit quality parameters are significantly higher in ecological plums, providing a compelling argument for adopting sustainable agricultural practices. These results highlight the importance of selecting sustainable agricultural practices, not only to safeguard the environment but also to ensure high-quality products suitable for human consumption.
listelement.badge.access-status Open Access ,
Biostimulant Effects of Algae Species, Arbuscular Mycorrhizal Fungi, and Their Combinations on Yield and Quality of Yellow Tomato Landrace Under Different Crop Cycles
(MDPI, 2024-08-19) Abidi, Soumaya; Tallarita, Alessio Vincenzo; Cozzolino, Eugenio; Stoleru, Vasile; Murariu, Otilia-Cristina; Abidi, Amina; Maiello, Roberto; Cenvinzo, Vincenzo; Lombardi, Pasquale; Cuciniello, Antonio; Hamrouni, Lamia; Caruso, Gianluca; Balti, Rafik
Recent agricultural research has prioritized the development of environmentally friendly management strategies to ensure food security, among which the application of biostimulants such as brown algae extracts, arbuscular mycorrhizal fungi (AMF), and their combination are included. The experimental protocol was based on the factorial combination of two planting times (4 May and 1 June) and seven biostimulant treatments (three brown algae species, Cystoseria tamariscifolia—C.t.; Fucus vesiculosus—F.v.; Padina pavonica—P.p.; arbuscular mycorrhizal fungi—AMF; C.t. + AMF; F.v. + AMF; P.p. + AMF) plus an untreated control. The earlier transplant resulted in a higher yield, due to the higher number of fruits per plant, and a higher plant fresh and dry biomass. The treatments with P.p. and F.v. extracts and the combination P.p. + AMF led to the highest yields (56.7 t ha−1), mainly due to the highest fruit number per plant. The earlier planting time led to higher values of dry residue, soluble solids, firmness, and colour component ‘a’. The highest values of fruit dry residue were recorded under the F.v. and P.p. extracts, and the combinations F.v. + AMF and P.p. + AMF, the highest soluble solid content with P.p. treatment, and firmness under P.p. + AMF. The highest levels of ‘L’ and ‘a’ fruit colour components were obtained under the P.p. extract treatment, of ‘b’ upon the application of P.p. and F.v. extract, and AMF + P.p. and AMF + F.v. The later planting time led to significantly higher values of the antioxidant parameters, as did the application of the P.p. extract and P.p. + AMF. CAT activity was more intense corresponding to the later tomato crop cycle, P.p. extract, and AMF + P.p. Overall, our study highlights the potential of biostimulants, particularly brown algae extracts and their combination with AMF, to improve tomato yield, antioxidant properties, and biochemical activities
listelement.badge.access-status Open Access ,
Effect of Foliar Selenate Supplementation on Biochemical Characteristics of PurslaneWeed (Portulaca oleracea L.)
(MDPI, 2024-07-04) Golubkina, Nadezhda; Amagova, Zarema; Kharchenko, Viktor; Bogachuk, Maria; Makarenko, Maria; Paleeva, Maria; Malinkin, Alexey; Andreeva, Katherine; Kavarnakaeva, Zulfia; Matsadze, Visita; Murariu, Otilia-Cristina; Caruso, Gianluca
The high biological activity of cultivated and wild purslane offers broad possibilities for utilizing this plant in medicine and human nutrition. To assess the prospects of obtaining new functional food products based on the wild form of P. oleracea L., foliar biofortification of this species with sodium selenate (VI) was carried out, and the changes in leaf and seed biochemical characteristics were investigated. Selenium significantly enhanced plant yield, photosynthetic pigments and the ascorbic acid content, and showed a tendency to seed productivity increase. The application of selenium augmented quinic acid content in leaves by 1.7 times but did not affect the oxalic acid content. Oxalic acid prevailed in wild purslane and quinic acid in cultivated purslane (cv. Makovey). Seed oil in Se-enriched purslane was characterized by a two-fold decrease in saturated fatty acids and squalene and 2.3-fold decrease in malonic dialdehyde content, along with a 1.4-fold increase in ascorbic acid. Selenium supplementation resulted in an increase in total lipids and mono- and di-unsaturated fatty acids and did not affect the concentration of ω-3 fatty acids and sterol accumulation. Among the identified sterols, only the minor ones (fucosterol, 7-stigmasterol and ∆7-avenosterol) showed a slight decrease upon Se supply. Compared to seeds of cv. Makovey, wild purslane seeds had higher levels of antioxidant activity by a factor of 2 and of polyphenols by a factor of 3.2 but did not differ significantly in oil fatty acid composition. The results indicate the importance of wild purslane leaves/seeds both fortified and not fortified with Se in human nutrition and medicine.
listelement.badge.access-status Open Access ,
Effects of Ripening Phase and Cultivar under Sustainable Management on Fruit Quality and Antioxidants of Sweet Cherry
(MDPI, 2024-07-08) Mineață, Iulia; Murariu, Otilia-Cristina; Sîrbu, Sorina; Tallarita, Alessio Vincenzo; Caruso, Gianluca; Jităreanu, Carmenica-Doina
Sweet cherry grown under sustainable management produces highly valuable fruits, whose quality shows important biochemical and morphological changes during ripening. Research was carried out in Iasi (Romania), with the aim to assess the quality characteristics of the sweet cherry fruits of three cultivars (Van, Andreiaș, Margonia), grown in an inner or outer position inside the tree crown, at the pre-ripening or full ripeness phase. In 2022, the colour component a* showed higher values in cv. Van and Andreias red fruits and in an inner position, whereas the components L* and b* at the full ripeness phase were highest in cv. Margonia. The dry matter and total soluble solids contents increased from the pre-ripening to the full ripeness phase and were highest in cv. Van sweet cherry fruits; the DM of fruit from the outer part of crown was higher than that of fruit from the inner part at the pre-ripening phase. The content of phenolics was the highest in cv. Margonia fruits at the pre-ripening stage and in cv. Van at the full ripeness phase and higher in the inner tree crown zones. The cultivar Margonia generally showed the highest vitamin C content in both years and development phases. The yellow fruit cv. Margonia mostly showed the highest values of chlorophyll a and b. The fruit’s content of carotene, lycopene, and anthocyanins was generally the highest in the red fruits of cv. Andreias. The examined sweet cherry cultivars showed a high variability in fruit nutritional quality and proved to be a rich source of bioactive compounds with antioxidant potential.
listelement.badge.access-status Open Access ,
Improvement of Physiological Features and Essential Oil Content of Thymus vulgaris after Soil Amendment with Chitosan Nanoparticles under Chromium Toxicity
(MDPI, 2024-06-20) Haghaninia, Mohammad; Rasouli, Farzad; Javanmard, Abdollah; Mahdavinia, Gholamreza; Azizi, Sahar; Nicoletti, Rosario; Murariu, Otilia-Cristina; Tallarita, Alessio Vincenzo; Caruso, Gianluca
An excessive amount of chromium in soil has detrimental effects on plant processes, and impairs food security, and public health. The application of nanoparticles may be a suitable solution and an innovative strategy by which to reduce plant abiotic stresses and pollution in the agricultural ecosystems. This research focuses on the effects of chitosan nanoparticles (CS-NPs) on thyme (Thymus vulgaris L.) plants grown in Cr-contaminated soil. The effects of CS-NPs as a soil amendment at four concentrations were investigated on plant nutrient uptake, photosynthesis parameters, antioxidant system, and essential oil (EO) content under soil Cr stress. The results show that chromium stress reduced fresh and dry weight of shoots, the uptake of macro-, and micro-elements, chlorophyll and carotenoids. The application of CS-NPs improved the antioxidant enzyme activity, reduced malondialdehyde, and increased the content of nutrients, EOs, photosynthetic pigments, and chlorophyll fluorescence parameters. The intermediate dose of chitosan nanoparticles (0.1% w/v) best valorized the content and yield of thyme EOs under chromium stress. These results are indicative that the application of CS-NPs can represent a supportive approach for plant production in soils contaminated with heavy metals.
listelement.badge.access-status Open Access ,
Effect of Seed Spaceflight Storage on Tomato Fruit Quality and Peel/Pulp Mineral and Antioxidant Distribution
(MDPI, 2024-03-18) Golubkina, Nadezhda; Dzhos, Elena; Bogachuk, Maria; Antoshkina, Marina; Verba, Olga; Zavarykina, Tatiana; Nechitailo, Galina; Murariu, Otilia-Cristina; Tallarita, Alessio Vincenzo; Caruso, Gianluca
The spaceflight storage of seeds is known to cause mutations affecting both their quality and the mature plants originating from them. To study the effects of space stress, tomato seeds of two cultivars (Lotus and Autumn rhapsody) were subjected to half a year of storage at the International Space Station (ISS), and then, sown in a greenhouse to produce tomato fruits. The space-treated plants gave smaller fruits with a stable total yield not significantly different from that of the control plants. Space-treated tomatoes showed significantly higher levels of dry matter, dietary fiber, monosaccharides and citric and malic acids and lower values of oxalic acid compared to the control plants. The pulp of space-treated fruits had 1.44–1.70 times lower levels of carotenoids, while their peel contained a 1.27–1.90 times higher pigment amount compared to the control plants. No significant changes in the total antioxidant activity (AOA), photosynthetic pigments and phenolic (TP) and proline content were recorded in the fruits due to seed spaceflight storage. Contrarily, space-treated tomatoes showed decreased levels of Ca, Sr and Mo and increased Se both in the fruit pulp and peel. The concentration of Fe and especially Pb was lower in space-treated fruit pulp. Positive correlations between Se and dry matter, Ca and Sr, Ca and Co, Ca and Fe, and Cr and carotenoids, and negative correlations between Se and Mo, Se and K, and Mo and dry matter were recorded. The results indicate that seed stress caused by long-term spaceflight affects both the biochemical characteristics and mineral composition of tomato fruits and causes the peel/pulp redistribution of carotenoids as well as macro- and micro-elements, improving Se accumulation levels in the fruit peel.
listelement.badge.access-status Open Access ,
Selenium and Heavy Metals in Soil–Plant System in a Hydrogeochemical Province with High Selenium Content in Groundwater: A Case Study of the Lower Dniester Valley
(MDPI, 2024-01-05) Sheshnitsan, Sergey; Golubkina, Nadezhda; Sheshnitsan, Tatiana; Murariu, Otilia-Cristina; Tallarita, Alessio Vincenzo; Caruso, Gianluca
The bioaccumulation of selenium (Se) and heavy metals (HMs) in plants is important because it can affect plant health and human nutrition. Recognizing the factors affecting Se accumulation in plants may have important implications for agricultural practices and human health in selenium-rich regions. The study primarily focused on the interactions between Se and HMs in the soil–plant system of the Lower Dniester Valley. Total concentrations of HMs (Cu, Mn, Zn) were determined by atomic absorption spectrometry, while Se concentrations were determined by a sensitive single-test-tube fluorometric method in solutions and extracts. Water-soluble Se (0.09 ± 0.03 mg·kg−1) in soils was 32.1% of the total Se (0.33 ± 0.13 mg·kg−1) and increased with the total rising Se content (r = 0.845). The results indicated that plants had a greater Zn accumulation capacity than that of the other HMs, suggesting its importance as a trace element for plant requirements. Se also had a high bioaccumulation rate. Se and Zn accumulation varied in different soil types, reflecting differences in bioavailability. In contrast, Mn and Cu showed low bioaccumulation, which varied with soil conditions and anthropogenic Cu pollution. Despite the Cu contamination of the soils in the investigated region, it can be inferred that the hydrogeochemical province with high Se content in groundwater has favorable conditions for Se mobilization in soils. The absence of antagonistic interactions with HMs in the soil–plant system contributes to the enhanced Se accumulation in plants in the Lower Dniester Valley. These results emphasize the complexity of the interactions between Se and HMs in the soil–plant system and their potential impact on agricultural practices.
listelement.badge.access-status Open Access ,
Essential and toxic elements analysis of wild boar tissues from North-Eastern Romania and health risk implications
(Frontiers Media, 2024-04-29) Boișteanu, Paul-Corneliu; Flocea, Elena-Iuliana; Anchidin, Bianca-Georgiana; Mădescu, Bianca-Maria; Matei, Mădălina; Murariu, Otilia-Cristina; Frunză, Gabriela; Postolache, Alina-Narcisa; Ciobanu, Marius-Mihai
Introduction: The level of essential minerals in meat is an important factor in human nutrition and health. Meat from responsibly managed wildlife is an alternative raw material with considerable nutritional benefits. Meat from hunted animals has essential and non-essential elements for the human body. It is important to carefully monitor the levels of heavy metals accumulated in the tissues of hunted animals in polluted areas to ensure food safety and environmental contamination. High levels of heavy metals in food and the environment can pose a danger to human health. Methods: The study aims to investigate the levels of essential mineral elements and heavy metals in the muscle tissue and organs of wild boar harvested through the herd density control plan over the last decade in north-eastern Romania. Results and discussion: The statistical analysis indicates that the age of the animals had a significant impact on Fe, Cu, and Zn levels in Longissimus lumborum. In the kidney a highly significant difference in Fe content by sex, with males showing higher values than females. The sex was shown to significantly influence the Mg levels. However, there are concerns about the accumulation of heavy metals such as Lead (Pb) and Cadmium (Cd), which may hurt the health of game meat consumers in the study area. Cd level it shows significant differences according to both age and sex, with higher concentrations in adults and males. Statistical analysis shows a negative correlation between Fe and Zn concentrations in muscle samples, while a positive correlation was found between Fe and Mn in kidney samples. There was also a positive association between Zn and Cu in muscle samples, but a negative association in kidney samples. Principal component analysis shows significant variation in essential element and heavy metals data between muscle and kidney samples. The loading plot shows a direct correlation between Pb and Cu and between Pb and Cd. However, an opposite correlation also is observed between Cu and Mg, Cd and Mg, and Pb and Mg. HQ (Hazard Quotient) for children compared to adults indicates a potentially higher risk associated with meat consumption among children because children are more vulnerable than adults. We report for the first time, to the best authors’ knowledge, various levels of essential minerals and exceeded maximum admitted level of heavy metals in the muscle tissues and kidneys of Sus scrofa ferus from Romania intended for human consumption, moreover, our findings highlight the need for strict monitoring and implementation of appropriate corrective measures, given the significant percentages of muscle and kidney samples exceeding the allowable limits for two of the most common toxic metals in the environment.
listelement.badge.access-status Open Access ,
Relevant Skills for Employment and Entrepreneurship in the Agri-Food Sector
(MDPI, 2024-01-16) Bodescu, Dan; Sîrghea, Alina; Rațu, Roxana-Nicoleta; Chiruță, Ciprian; Mălăncuș, Răzvan-Nicolae; Donosă, Dan; Robu, Alexandru-Dragoș
In the current social, economic, natural and geopolitical context, there is an acute need for research on professional and entrepreneurial skills in line with the challenges and opportunities of the rapidly changing global economy. The aim of this study was to determine the skills required by employees and entrepreneurs in the agri-food sector. This research study consisted of interviews, questionnaires and focus groups on a sample of 111 employers, 288 students and 139 teachers from the North-East Development Region of Romania. The most important skills needed by higher education graduates in the agri-food sector were communication, learning and social skills, with values of 90.5, 74.3 and 70.6 points, respectively. The least appreciated skills were cultural, linguistic and mathematical skills, with values of 17.5, 36.9 and 43.8 points. For developing and running an entrepreneurial activity, the subjects appreciated communication (91.0 points), as in the case of employment-related skills, followed by economic skills (81.4 points) and learning skills (75.4 points). Additional efforts are needed to increase the relevance of practical activities in correlation with the skills required by the market, and it is necessary to strengthen the current partnerships and create new partnerships between universities and the economic environment by employing public and research entities.
listelement.badge.access-status Open Access ,
The development of value-added yogurt based on pumpkin peel powder as a bioactive powder
(Elsevier, 2024-03-11) Rațu, Roxana-Nicoleta; Cârlescu, Petru-Marian; Veleșcu, Ionuț-Dumitru; Arsenoaia, Vlad-Nicolae; Stoica, Florina; Stănciuc, Nicoleta; Aprodu, Iuliana; Constantin, Oana-Emilia; Râpeanu, Gabriela
This study aimed to explore the utilization of pumpkin by-products as a bioactive powder in the development of value-added yogurt. Pumpkin peel resulting from food processing contains antioxidant components like phenolics, flavonoids, and carotenoids, compounds characterized by high bioactivity. This study investigated the potential of incorporating pumpkin peel powder (PPP) into yogurt to enhance its nutritional value and sensory attributes. Results indicated that the incorporation of PPP into yogurt resulted in improvements in nutritional composition, particularly in terms of β-carotene and bioactive compounds. Additionally, the addition of PPP positively influenced the textural properties of the yogurt. The sensory evaluation revealed that the incorporation of pumpkin peel had no negative impact on the overall acceptability of the yogurt, with some samples (YPP2) even exhibiting preferred sensory characteristics compared to the control. The utilization of PPP as a bioactive powder in yogurt presents a promising strategy for reducing food waste and creating innovative, value-added dairy products. The development of such products can not only contribute to sustainable food production but also provide consumers with more diverse food choices with enhanced characteristics.

Browse

Recent Submissions