2023

Permanent URI for this collectionhttps://repository.iuls.ro/handle/20.500.12811/4425

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listelement.badge.access-status Open Access ,
The management of arthritic pain in dogs– a review
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Neagu, Daniela; Leblond, Clarisse; Biriș, Alexandra; Codea, Andrei-Răzvan; Popovici, Cristian; Mureșan, Alexandra-Nicoleta
Osteoarthritis is a common condition in dogs, particularly affecting elderly individuals, and the chronic pain it causes significantly impacts the quality of life of affected dogs. First, we will focus on the joint, the physiopathology of osteoarthritis, and the mechanisms of arthritic pain production, and then discuss the existing treatments. There are numerous treatments available for managing this complex osteoarticular condition, but unconventional therapies are increasingly of interest to owners of canine species. Phytotherapy harnesses the healing properties of plants for treatment in a less toxic, more natural, and more cost-effective manner, offering a wide range of therapeutic options for animals. The objective of this review is to evaluate the present evidence backing treatments for canine osteoarthritis. This includes non-steroidal anti-inflammatory drugs, piprants, monoclonal antibodies, adjunctive analgesics, structuremodifying osteoarthritis drugs, phytotherapy, and regenerative therapies.
listelement.badge.access-status Open Access ,
Effects of antioxidant treatment on cell differentiation in rabbit embryos
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Torda, Iuliu; Spătaru, Ioana-Irina; Boldura, Oana-Maria; Lungu, Bianca-Cornelia; Marc Zarcula, Simona; Bratu, Daniel; Otavă, Gabriel; Huțu, Ioan; Mircu, Călin
The antioxidant coenzyme Q10 can influence the expression of genes involved in apoptosis and energy metabolism of oocytes and quercetin can improve oocyte maturation and early embryonic development. In this study, the gene expression of GATA6 and NANOG in rabbit embryos was assessed using the qRT-PCR reaction. The groups were: group A- control group (no treatment added), group B (hormonal treatment of superovulation, which included the administration of PMSG and hCG), group C (administration of quercitin) and group D (administration of Coenzyme Q10). Our results show that the expression of the two genes was different depending on both the stage of embryonic development and the treatment administered. The highest values of gene expression for GATA6 and NANOG were obtained in groups 2, 4, 7, 8 and 9, corresponding to morula and blastocyst stages. In addition to the fact that NANOG and GATA6 are factors that are involved in early embryonic development, we believe that the administration of extrapituitary gonadotropins and antioxidants contributed to the increase in gene expression.
listelement.badge.access-status Open Access ,
Synchronization of ovulation (FTET) in Țurcana sheep as embryo recipients
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Ciornei, Ștefan-Gregore; Șchiopu, Sebastian-Gabriel; Pruteanu, Ioana-Livia; Drugociu, Dan-Gheorghe; Nechifor, Florin; Roșca, Petru
Due to the fact that the farm wants to crossbreed sheep with meat breeds, it was opted for the fastest solution to bring genetic progress, namely embryo transfer, using sheep from the Turcan breed as embryo recipients and those from the Suffolk breed as embryo donors, so that finally the batch of receivers after parturition will produce a production of Suffolk lambs. Following the selection of recipients considering the standard criteria that include: general health, functional integrity of the genital tract and cyclic activity of the ovaries, two groups were formed for the induction and synchronization of estrus. The first batch consisted of 20 sheep and the second batch of 20. The first batch was subjected to a P4-PG-PMSG protocol and the second batch to the P4-PG-GnRH protocol. The two protocols consisted of the insertion of intravaginal sponges with flugestone acetate in a concentration of 20mg, to induce the progesterone phase, for 13 days, day 0 of the protocols being represented by the day when the progesterone sponges were applied. In continuation of the protocol, Prostaglandinf2α was used on day 12 in a double dose, in the morning and in the evening at a distance of 12 hours in a dose of 0.6 ml/administration/animal. On day 13, the progesterone sponges were removed and PMSG (Folligon) was administered in the case of the first batch at a dose of 600 IU/animal, and in the second batch GnRH (Receptal) was administered at a dose of 12 μg/animal ( 3ml/animal) followed by a dose of 600 IU HCG (Chorulon) 24 hours after GnRH administration. Ovulation was accurately assessed at the time of embryo transfer through laparotomy, due to the highlighting of the ovaries and their macroscopic analysis in the operative field, after identifying the CL on the ovary, their appearance and the number of CL on the ovary. However, the assessment of the rate of entry into heat was assessed 24 hours after the end of the protocol by biostimulating the receptors with the help of detector rams, thus the ewes that entered in estrus at 12 – 24 hours, 24 – 48 of hours or over 48 hours after completing the protocol. The results obtained in the case of the first batch having the highest rate of entering in estrus between 24 - 48 hours being 40%, followed by a rate of 30% between 12 - 24 hours, the lowest rate being 20% that entered in estrus after 48 hours, the second batch with GnRH had the highest rate of entry into estrus of 60% in the first 12-24 hours and the rate at 24-48 hours, respectively those that entered heat after 48 hours was 20%. Compared between the two groups, there were differences in the timing of ovulation assessment, as in the case of the first group with PMSG, a 60% ovulation rate was assessed with well-developed CL, 10% presented CL but these were unsuitable for embryo transfer, 10% presented ovarian cysts, and 10% did not ovulate, in the case of the second batch an ovulation rate of 60% was assessed and the rate of 40% represented the animals that did not ovulate, the difference between the two batches being the fact that it is observed in the case of the first batch rate of 10% with ovarian cysts, which indicates that the PMSG-based pharmaceutical is causing ovarian cysts. In conclusion, the therapeutic protocol used in the off-season for the induction of estrus and ovulation in Turcan sheep, in this study, resulted in the detection of estrus in 90% of the ewes subjected to the protocol, and the ovulation rate was 80% (60% with CL well developed and 20% were with poorly developed CL), regarding the first batch, and regarding the second batch 100% of ewes in oestrus were detected, but 60% of them ovulated with CL well developed. These results can be largely attributed to the reproduction seasonality of the sheep.
listelement.badge.access-status Open Access ,
Detection of beta-lactam residues in environmental and drinking water by immunoenzymatic assay
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Gașpar, Cristina; Lăzărescu, Cristian; Grigoreanu, Alexandra; Bucur, Iulia-Maria; Tripon, Roberta; Tulcan, Camelia
In recent decades, concern over emerging organic contaminants in the environment has grown considerably because of their potentially harmful effects on organisms and ecosystems. These synthetic compounds are widely used in modern life-style and due to improvements in analytical technologies, we are now able to identify and quantify them even in small concentrations. One of the most important pharmaceutical contaminants is antibiotics, of which more than half belong to the class of beta-lactams. This research aimed to determine the beta-lactam antibiotics residues in surface water (rivers) and groundwater, which serve as public or private sources of drinking water, as well as in urban wastewater. The samples were collected from different places throughout the Western part of Romania and analyzed using commercially available ELISA kits for the detection of beta-lactams in liquid samples. The results show that betalactam antibiotics are ubiquitous in all categories of water and establish the ELISA method as an acceptable screening tool for antibiotic residues.
listelement.badge.access-status Open Access ,
Proactive sanitary-veterinary monitoring of bee families included in a prophylaxis program (Active beekeeping season 2023)
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Savu, Vasilică; Șapcaliu, Agripina; Fătu, Viorel
The purpose of this work is to monitor the state of bee health through morphoclinical and laboratory examination of bees on samples of live bees and honeycombs with brood for the prevention and control of diseases in bees in order to include them in a study on the impact of the non-ionizing electromagnetic radiations on bees. Samples were collected during the active season 2023, and morpho-clinically and laboratory examined according to OIE regulations from 9 private apiaries (PH, AG, TR, CL, VN, IS, DB, VL and IF) from which we collected 18 samples. The laboratory results revealed the existence of some diseases in 6 apiaries (66.67%), as follows: a unique evolution (suspected foulbrood disease in one apiary) (11.11%), five apiaries with mixed conditions (55,56%) (varroosis, nosemosis, chalkbrood, and suspected poisoning), and three apiaries were diagnosed as clinically healthy (33.33%). Studying the influence of nonionizing radiation on bees has wider implications for ecology and the environment, as bees play a crucial role in pollination and maintaining ecosystems. The interaction between bees and electromagnetic radiation is a complex and multifactorial issue that may explain the diversity of conclusions in the available studies. Therefore, studies are needed in Romania to better understand the connection between non-ionizing electromagnetic radiation and the decline of bee populations (Collony Colapse Disorder).
listelement.badge.access-status Open Access ,
The importance of canine pancreatic lipase in relation to ultrasound examination
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Blăgeanu, Andrei; Vulpe, Vasile
Pancreatitis represents the most often met pathology of the exocrine pancreas. It can be acute or chronic depending on the percentage of lesions to the parenchyma. The causes of the pathology aren’t fully understood yet but it has been observed that inadequate nutrition has a really important role. Some breeds are thought to be more predisposed to this pathology such as: Yorkshire Terrier, Cocker, Dachshund. The clinical signs can range widely; many patients are subclinical (especially the ones with chronic pancreatitis), while others can have a variety of gastro-intestinal signs. We can divide this signs into characteristic ones (such as vomiting, diarrhea, apathy, abdominal pain) and uncharacteristic signs (such as ascites, jaundice, fever). Seeing the complexity of this pathology, just a simple clinical examination won’t help us diagnose it, but will give us clues for what paraclinical exams to take. This article will present the correlation between ultrasound imagistic findings considered to be the main tool for examination of the pancreatic structure and canine pancreatic lipase (also known as CpL), considered to be the golden standard for detecting pancreatic disorders.
listelement.badge.access-status Open Access ,
When gallbladder oedema comes as a chronic disease – diagnosis and treatment
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Blăgeanu, Andrei; Vulpe, Vasile; Ifteme, Constantin-Vasile
Diagnosis of the gallbladder oedema is difficult during the clinical examination because the clinical signs are not specific for this disease. The causes of this pathology are multiple and can be divided into emergencies (such as anaphylactic shock, right-sided hearth failure, pericardial effusion) and chronic pathologies (such cholecystitis, pancreatitis or immune-mediated hemolytic anemia). Imagistics methods complete the information of the clinical exam and ultrasound is considered the golden standard for diagnosing this pathology. Normally, at the ultrasound exam, the gallbladder has a hyperechoic wall, with a diameter of 2-3 mm; while in case of gallbladder oedema, the wall is thick and has a triple stratification, with 2 hyperechoic rows, separated by a hypoechoic line. This pathognomonic description of the gallbladder oedema is also known as the ‘Halo Sign’ or ‘Double Rim Effect’. Making a correct differential for the appearance of the sign is vital for the outcome of the case. The main focus is to exclude every emergency cause in order to start treating the chronic pathologies. This paper presents the pathologies leading to gallbladder oedema, met at the Radiology and Emergencies Departments from the Faculty of Veterinary Medicine Iasi during the period march 2020 – may 2022. The clinical case presented was diagnosed and treated at the Center of Endoscopy and Minimally Invasive Surgery Bucharest.
listelement.badge.access-status Open Access ,
Impact of A2 milk on human health and the dairy industry - a review
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Ariton, Adina-Mirela; Neculai Văleanu, Andra-Sabina; Poroșnicu, Ioana
In recent years, a new type of cow's milk, called "A2 milk," has been introduced to the market. This type of milk was first marketed in New Zealand and has since gained a presence in the markets of several countries. It mainly contains two types of β-casein: the A1 and A2 variants. In recent years, researchers have studied the possible implications of the composition of the β-casein protein fraction for the manifestation of a new intolerance: milk protein intolerance. Casein is the main component of milk proteins, of which approximately 30-35% is beta-casein. A2 beta-casein has proline at position 67 of the protein amino acid chain, while A1 beta-casein has histidine at that position; this is associated with a possibility of gastrointestinal discomfort due to β-casomorphin-7 (BCM-7) released during gastrointestinal digestion. The purpose of this review is to provide an update on the impact of A2 milk on human health as well as on its many technological qualities for the production of dairy products with improved health benefits for consumers.
listelement.badge.access-status Open Access ,
Association between foot skin temperature (FST) and locomotion scoring (LS) in dairy cattle
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Neculai Văleanu, Andra-Sabina; Ariton, Adina-Mirela; Sănduleanu, Cătălina; Poroșnicu, Ioana; Radu, Ciprian
The health status of the hoof in dairy cattle is crucial for their overall well-being and productivity. Hoof diseases and lesions can lead to lameness, decreased milk production, and economic losses for dairy farmers. Traditional methods of assessing hoof health involve manual inspection and trimming, which can be time-consuming and subjective. This research article explores the potential use of a phone-connected infrared camera as a non-invasive and objective tool for assessing the health status of the hoof in dairy cattle, by investigating the association between foot skin temperature (FST) and locomotion scoring (LS) in a group of Romanian Black Spotted dairy cows. LS was carried out using the mobile app developed by the Wisconsin University (Locomotion Scorer). The thermograms were collected during afternoon milking and processed using the FLIR software. Overall, 73.9% of the cows were scored as non-lame, while 13.04% presented signs of foot lesions. The highest temperature observed in the interdigital area, in the lame group, by thermography, was T°=36.5°C. In conclusion, monitoring hoof health status in dairy cattle is essential for ensuring their well-being and productivity. The use of an infrared thermal camera for the assessment of foot surface temperature has shown promise as a noninvasive tool for evaluating hoof health.
listelement.badge.access-status Open Access ,
Goniometric measurements of the femoral joint in dogs with hip dysplasia
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Patrichi, Teodora-Sonia; Lucaci, Felix-Daniel; Costin, Anamaria; Lăcătuș, Caroline-Maria; Mârza, Sorin-Marian; Purdoiu, Robert-Cristian; Musteață, Mihai; Lăcătuș, Radu
Canine hip dysplasia is one of the most common orthopedic dieseases presents in most breeds of dogs but more prevalent in large breeds. Clinical examination through tests such as Ortolani, Barlow, and Bardens, alongside with radiological, tomographic, magnetic resonance, and ultrasonographic examinations, are the only methods for screening and diagnosing canine hip dysplasia. Canine hip dysplasia can lead to other musckuloskeletal diseases, with the most common ones being cruciate ligaments tear and spinal conditions such as IVDD or degenerative myelopathy. To assess hip changes, it is recommended that the patient to be heavily sedated, and the standard exposure will be with the hips in forced extension. Among knee conditions related to hip dysplasia, the most commonly reported are patellar luxation, partial cranial cruciate ligament rupture, and osteoarthritic changes. A study conducted on 65 dogs found concurrent hip dysplasia and patellar luxation in 28% of cases. Clinical goniometry is an objective and non-invasive method of measuring joint angles, allowing for the assessment of the normal range of joint movements. This method can indicate the severity of joint pathology. Currently, goniometry is often used in the field of imaging and it can be an important element in assessing the musculoskeletal system. The aim of this paper is to evaluate the effectiveness of goniometric measurements at the knee and hip joint levels in dogs with hip dysplasia and if there is a correlation. A total of 10 dogs from varying breeds of dogs aging from 1 to 3 years old, were deeply sedated in order to measure perform the x-rays in ventro-dorsal hip extended view for measuring the Norberg angle (Na), anatomic Latero-Proximal Femoral Angle (aLPFA), anatomic Latero-Distal Femoral Angle (aLDFA), mechanical Latero-Distal Femoral Angle (mLDFA), mechanical Latero-Proximal Femoral Angle (mLPFA). All patients were part of the clinical cases present in our department and the written consent of the owners was obtained for each dog. Norberg and femoral angles were measured within the local DICOM viewer and the statistical analysis was performed with IBM SPSS Statistics. All images were reviewed by an ECVDI resident, a radiology professor and a radiology intern. All patients within our study had a Na under the reference value of 105º. All the femoral angles were within limits regarding their normal values. We conclude that there is not a signicative relation between the Na and the goniometric values obtained, most likely because of other factors that are influencing these measurements (ex. breed variations, femoral slightly rotation, muscle contracture due to poor anesthesia management, other musculoskeletal genetic disorders etc.). Further research on a more relevant statistical sample is recommended, in order to determine the normal goniometric values and the changes that appear in case of hip dysplasia.
listelement.badge.access-status Open Access ,
Effects of lumbar intervertebral disc herniation on adjacent musculature on computed tomography (CT) examination
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Voiculeț, Claudiu-Ionut; Văleanu, Cristian-Ioan; Lucaci, Felix-Daniel; Costin, Anamaria; Timofte, Robert-Emilian; Lăcătuș, Caroline-Maria; Mârza, Sorin-Marian; Purdoiu, Robert-Cristian; Lăcătuș, Radu
In the late 1800s Dexler used for the first time the term IVDD (Intervertebral disc disease), which was described as the presence of cartilaginous material in vertebral canal. Intervertebral disc herniation (IVD) refers to the part of the disc that is within vertebral canal. The aim of the paper was to highlight the morphometric changes of the dorso-lumbar musculature occurring in dogs diagnosed with the presence of hyperattenuating material in vertebral canal by CT. Thirteen dogs from varying breeds of dogs (French bulldog, Dachshund, Bichon, Pekingese) age from 2 to 8 years old, with hind limbs locomotory disease were scan with a Somatom Scope 16-slices CT scan. Results: Pronounced muscle contracture was seen on CT scan thus: 1,4 mm (+-0,6mm) in Bichon on left multifidus muscle in the area of the protrusion, 1.6 mm (+- 0,6mm) in Dachshund on left multifidus muscle in the area of the protrusion, 2,1 mm (+- 3 mm) in Pekingese on left multifidus muscle in the area of the protrusion and 1,2 mm (+- 0,5 mm) in French bulldogs on the right multifidus muscle in the area of the protrusion. An increased muscle contracture was seen in all patients in the area of the herniation.
listelement.badge.access-status Open Access ,
Tailored management plan for preventing viral diseases within dog shelters
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Vasilache, Florin-Jan
Dog shelters play a crucial role in animal welfare by providing temporary care and finding permanent homes for homeless dogs. However, the close confinement and high turnover of animals in shelters create an ideal environment for the spread of viral diseases. To combat this, various preventive measures have been implemented, but a comprehensive and tailored approach is essential to effectively safeguard the health of shelter dogs. This study outlines a tailor-fit management plan that incorporates a multifaceted approach, encompassing vaccination, testing, hygienic practices, and quarantine protocols, to effectively prevent viral diseases within dog shelters.
listelement.badge.access-status Open Access ,
Haematological changes in canine parvovirus infection
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Dascălu, Mihaela-Anca; Bocăneți Daraban, Florentina; Manole, Gabriel; Cozma, Andreea-Paula; Moroșan, Șerban; Tanase, Irina-Oana
Canine parvovirus (CPV) infection is a highly contagious disease caused by canine parvovirus type 2 and commonly produce acute gastrointestinal illness. All dogs are susceptible to CPV, although some dogs are at greater risk than others, as puppies (between 6 weeks of age and 6 months), unvaccinated or incompletely vaccinated dogs. Due to the virus multiplication and effect on the bone marrow, severe haematological changes are reported, resulted also from the combination of severe inflammation, gastrointestinal bleeding and depletion of hematopoietic cell lines. In the present study, haematological changes in dogs diagnosed with CPV infection were analysed.
listelement.badge.access-status Open Access ,
Therapeutic alternatives in mammary gland infections in cows
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Soreanu, Dumitru-Octavian; Tanase, Irina-Oana; Bocăneți Daraban, Florentina; Dascălu, Mihaela-Anca; Țuțu, Paul; Mareș, Mihai
Mastitis caused by infectious pathogens is still considered a ravaging disease of dairy cattle, affecting animal welfare. Economically, this condition appears in the dairy industry through reduced production performance and increased culling rates. Bovine mastitis is a mammary gland inflammation, most commonly caused by bacterial pathogens. Routine diagnosis is based on detecting clinical and subclinical forms of the disease. This highlights the importance of rapid detection of etiological agents at the farm level, for which several diagnostic techniques have been developed. Due to the predominance of bacterial etiology, treatment in mastitis is mainly based on the use of antibiotics. Nevertheless, antibiotic therapy has some limitations due to antimicrobial resistance, treatment efficacy, and costs at the farm level. Research needs to be directed toward developing new therapeutic agents/techniques that can replace conventional methods and address the problem of antibiotic resistance. The objective of the article is to briefly describe the current findings and results of herbal therapy as an adjuvant in the management of mammary gland infections.
listelement.badge.access-status Open Access ,
Classification and clinical significance of papillomavirus infection in domestic cats
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Țuțu, Paul; Tanase, Irina-Oana; Bocăneți Daraban, Florentina; Dascălu, Mihaela-Anca; Soreanu, Dumitru-Octavian; Mareș, Mihai
During the last decades, the infection with papillomavirus in domestic cats gained interest from the veterinary community due to its significant impact on the companion's animal's health. Therefore, in this review, we aim to present a concise classification of feline papillomaviruses and their clinical relevance in domestic felines. Initially, the different types of papillomaviruses affecting domestic cats are described. Here, we emphasize the molecular diversity and transmission ways to better understand each virus type and its clinical implications. Furthermore, we explore the clinical importance of papillomavirus infections, analyzing their various manifestations such as skin or oral lesions. We outline the signs and symptoms of these infections, shedding light on the oncogenic mechanisms used by the virus. The knowledge gained from this analysis holds the potential to refine veterinary medical practices, enabling the effective management of this condition and ultimately enhancing the overall quality of life for our feline companions.
listelement.badge.access-status Open Access ,
Food safety in the age of transparency: clean label products in the post-Covid-19 era
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Crivei, Ioana-Cristina; Crivei, Luciana-Alexandra; Cozma, Andreea-Paula; Veleșcu, Ionuț-Dumitru; Rațu, Roxana-Nicoleta; Stoica, Florina; Postolache, Alina-Narcisa
Clean-label products are defined as foods and beverages formulated with simple, natural, and familiar ingredients, instead of using artificial ingredients and additives. The clean label movement has gained popularity because of a rise in consumer demand for authentic, transparent, and healthier foods and beverages. In the post-COVID-19 era, the clean label trend has evolved considerably, indicating a shift in consumer preferences and demands. The COVID-19 pandemic has had a significant effect on consumer behavior, particularly regarding food safety and products with clear labels. According to recent studies, during the pandemics, consumers have shown an increased interest in products with clean labels as they seek healthier choices. In addition, the pandemic has impacted consumer purchasing patterns, with a shift toward ingredient examination and an increased demand for healthful products. In conclusion, the COVID-19 pandemic has highlighted the significance of the clean label trend, redirecting consumers toward safe, natural, and transparent food products, and emphasizing the importance of local purchasing and sustainable supply chains.
listelement.badge.access-status Open Access ,
Prevalence of infection with Dirofilaria immitis and Dirofilaria repens in dogs from the South-Eastern Part of Romania
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Matfei, Anca; Ivănescu, Maria-Larisa; Andronic, Bianca-Lavinia; Mardare Mîndru, Raluca; Martinescu, Gabriela-Victoria; Acatrinei, Dumitru-Mihai; Iacob, Olimpia; Miron, Liviu-Dan
As a result of global warming, the change in the biological cycle of vectors and the increase in intercontinental travels, we are now facing an increase in the number of cases of vector-borne diseases. Among these, heartworm disease has escalated in recent years in Romania, with cases increasing in many parts of our country. Our study was carried out in the South-Eastern part of Romania, with the aim of establishing the prevalence of heartworm disease in dogs, with the identification of the two species Dirofilaria immitis and Dirofilaria repens. Blood samples were collected during the period May - July 2022 and January - July 2023, from 220 dogs with and without an owner from Tulcea county, from places such as Tulcea, Murighiol, Somova, Mineri, Niculițel. The blood samples were tested through the Knott method, which is considered as the reference method in the diagnosis of dirofilariosis in dogs. The results show a prevalence of Dirofilaria spp. infection of 11.36% in dogs. Of the 220 samples examined by the Knott test, 25 were positive for circulating microfilariae, of which 21 showed infestations with Dirofilaria immitis and 4 with co-infection with Dirofilaria immitis and Dirofilaria repens. The study underlines the importance of introducing monitoring and control programs for heartworm disease in dogs in Romania.
listelement.badge.access-status Open Access ,
The prevalence of intestinal parasites in dogs from shelters in Constanța County-Romania
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Rusu, Andrada-Hermina; Ivănescu, Maria-Larisa; Martinescu, Gabriela-Victoria; Acatrinei, Dumitru-Mihai; Miron, Liviu-Dan
This study was designed to evaluate the prevalence of gastrointestinal parasites in shelter dogs from Constanța, a southeastern county from Romania. In order to assess, in 2022 and 2023, individual and pooled fecal samples, were collected from 611 dogs from 9 shelters. Fecal samples were examined using standard flotation and sedimentation methods. The overall prevalence of gastrointestinal parasites was 74.63%. Eggs of hookworms (Ancylostoma sp. and Uncinaria sp.) were the most frequently detected (55.97%), followed by Cystoisospora sp. (31.91%), Trichuris vulpis (27.33%), Toxocara canis (21.27%), %), Capillaria sp. (2.78%) and Toxascaris leonina (1.96%). Cases of single infestation were found in 217 (35.51%) of the tested dogs. Mixed infestations with two or more species of parasites were observed in 239 samples, representing 39.11% of the total samples analyzed. These results will be useful for establishing health care programs in dog shelters and for implementing effective strategies in controlling the intestinal parasites, in order to restrain the spread of zoonotic parasites and to raise awareness of their impact on public health.
listelement.badge.access-status Open Access ,
Researches regarding the paraclinical correlations between urinalysis and water intake in cats with feline urologic syndrome
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Vițălaru, Bogdan-Alexandru; Ștefănescu, Alina
The research aimed the direct correlations between the water composition and feline urological syndrome prognosis. The study was conducted on 60 patients aged between 1 and 10 years divided into two batches of 30 each. The first batch (LOT1) was represented by 30 oliguric patients, with a value of urinary specific gravity (USG) >1.055, an acidic pH and a urinary protein/creatinine ratio (UPC) >0.2, and the second batch (LOT2) was represented by 30 polyuric patients, with a urinary specific gravity (USG) <1.035, a neutral or alkaline pH and a urinary protein/creatinine ratio (UPC) <0.2 or borderline (0.2-0.4). In the first batch (LOT1), alkaline water without sodium and potassium, was administered. The water was based on a salt-free formula with a pH of 8.0 and 10 ppm of potassium. In the second batch (LOT2), a neutral water with potassium was used. The water was based on a formula with salts (magnesium citrate, calcium acetate and sodium bicarbonate), with a pH of 7.0 and 12.5 ppm potassium. In both batches, the evaluation of the USG, the pH and the UPC was carried out for 180 days, at 30, 90 and 180 days. Hydration is an essential component in the management of patients diagnosed with feline urological syndrome. Administering an alkaline hydric diet in patients with aciduria is a solution to counteract the effect of metabolism on urinary pH. Potassium supplementation in polyuric patients is a beneficial solution in hypokalemia therapy. Potassium restriction in oliguric patients is a beneficial solution in the therapy of hyperkalemia.
listelement.badge.access-status Open Access ,
Acute kidney injury in a dog diagnosed with leishmaniasis: case report
("Ion Ionescu de la Brad" Iași University of Life Sciences, 2023) Ștefănescu, Alina; Florea, Cristian-Ionuț; Boancă, Crina-Alexandra; Rădulescu, Andrei; Vițălaru, Bogdan-Alexandru
A 3 years old, 40.8 kg, intact male American Bully, diagnosed with acute kidney injury in a private clinic in Serbia was referred for hemodialysis therapy. The dog was presented with the following symptoms: lethargy, appetite loss, vomiting, diarrhea, weight loss, dehydration (8-10%, Considerable loss of skin turgor), rectal temperature of 38.8oC and dry mucous membranes. Arterial blood pressure was elevated 218-220 mmHg systolic, using Doppler method. The biochemistry revealed elevated ALT 191 (RR: 10-118 U/L), AMY 1390 (RR: 200-1200 U/L), BUN 94 (RR: 7-25 mg/dL), CREA 3.6 (RR: 0.4-1.2 mg/dL), PHOS 9.8 (RR: 2.9-6.6 mg/dL), GLU 121 (RR: 60-110 mg/dL), K 3.3 (RR: 3.4-5.6 mmol/L). Urine analysis was performed with UPC 0.2-0.5 (borderline proteinuric), pH 5.5, microalbumin >25 mg/L, creatinine >26.4 mmol/L. The infestation with Leishmania infantum was confirmed using quantitative PCR. The patient was stabilized using fluid therapy and parenteral feeding. Hemodialysis was decided as an extracorporeal replacement therapy for sustaining renal function. A central venous catheter was placed under a light sedation with oxygen therapy. Hemodialysis was performed for three times in a period of 11 days. A key treatment for acute kidney injury in canine patients is represented by intensive care, fluid therapy and hemodialysis therefore, the values were reduced in BUN from 94 mg/dL to 30 mg/dL and CRE from 3.6 mg/dL to 1.8 mg/dL. The BUN and CREA reached normal values 39 days after that the patient was discharged.

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