2019
Permanent URI for this collectionhttps://repository.iuls.ro/handle/20.500.12811/5106
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listelement.badge.dso-type Item , listelement.badge.access-status Open Access , Ultrastructural aspects of yersinia ruckeri cells after treatment with non-thermal plasma-activated water(Revista de Chimie SRL, 2019-02-15) Lupu, Andrei-Cristian; Bostănaru, Andra-Cristina; Mareș, Mihai; Ursu, Laura; Roman, Constantin; Mardare Mîndru, Raluca; Miron, Liviu-DanYersinia ruckeri is the causative agent of enteric red mouth disease (ERM, yersiniosis), one of the most important diseases that affects particularly farmed salmonids species. Numerous articles have demonstrated that Y. ruckeri can cause both epizootics and zoonosis. Y. ruckeri shows the ability to survive outside the host in nutrient-limiting environments for long periods due to biofilms forming capacity with adherence to solid supports but also for the adherence to the host tissues. Considering these aspects, the control of Y. ruckeri can be a problem, because of its resistance. Recently, non-thermal plasma activated water (PAW) proved to be active against Gram-negative bacteria and this fact could be also useful in Y. ruckeri control. The purpose of this in vitro study was to test the antimicrobial efficacy of PAW against Y. ruckeri and to explore the ultrastructural changes in these bacteria. Ultrastructural changes in Y. ruckeri cells, probably related to the action of PAW, included modifications in the shape and texture of the outer membrane. These changes in the bacterial membrane have been linked with the inactivation of bacteria by PAW exposure.listelement.badge.dso-type Item , listelement.badge.access-status Open Access , Conditioning of praziquantel and florfenicol for some heterologous coinfections of farmed carp(Revista de Chimie SRL, 2019-08-15) Lupu, Andrei-Cristian; Bomboș, Mihaela; Barbacariu, Cristian-Alin; Roman, Constantin; Mardare Mîndru, Raluca; Martinescu, Gabriela-Victoria; Miron, Liviu-DanCoinfections can alter the course and severity of different fish diseases. Suspensions of praziquantel (PZQ) and florfenicol (FFC ) based on sunflower oil can be an effective alternative for treating fish coinfections. The use of polyoxyethylene (20) sorbitan monooleate in the presence of ethoxylated castor oil in the preparation of PZQ and FFC suspensions has improved the stability of these suspensions. This PZQ and FFC preparation can be used in the treatment of parasitic/bacterial heterologous coinfections. The suspension of the PZQ and FFC preparation can be administered by water-diffusion, having a controlled release. The compounds are biodegradable and the route of administration remains safe for the environment.listelement.badge.dso-type Item , listelement.badge.access-status Open Access , Serological study of exposure to leishmania in dogs living in shelters, in South-East Romania(Asociatia Generala a Medicilor Veterinari din Romania, 2019) Cîmpan, Andrei-Alexandru; Diakou, Anastasia; Papadopoulos, E.; Miron, Liviu-DanOne of the most important canine vector-borne diseases in the Mediterranean region is canine leishmaniosis and there are frequent reports that it is spreading to the North and East of Europe. Romania is one of the countries traditionally considered non-endemic. However, L. infantum infections in dogs, humans and one jackal, as well as vector-competent sandflies have been recently reported in the country. To determine the seroprevalence of this infection we collected sera from 110 stray dogs, from two counties (Galați and Călărași), from the south-east of Romania and investigated for the presence of IgG antibodies against Leishmania by an in-house ELISA. Anti-Leishmania antibodies were detected in 5 stray dogs from the county of Galați. The prevalence in the county of Galați was 8.33% and the overall prevalence was 4.54%. These results are comparable to what was reported before in the southern county of Vâlcea and suggest that stray dogs from the south of Romania are exposed to L. infantum. There is a need for more studies in order to understand the situation of L. infantum infections in Romania.