2016

Permanent URI for this collectionhttps://repository.iuls.ro/handle/20.500.12811/5109

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listelement.badge.access-status Open Access ,
Inclusion complex of thiotriazinone with α-cyclodextrinraman spectroscopy, DSC, preliminary antimicrobial and antifungal study
(Forum of Chalcogeniders, 2016-03-04) Ghiorghiasa, Ramona; Petrovici, A.R.; Roșca, I.; Miron, Liviu-Dan
Thiotriazinone (TTZ) is used as an intermediate of cephalosporin, but very few things are known about its activity. The purpose of this study was to form a complex of Thiotriazinone-α-cyclodextrine and to investigate its antimicrobial activity, in order to use this ”smart drug” in animal diseases therapy. The complex was made by liophlization at a molar ratio TTZ-αCD=1:1. Characterization was made by Raman spectroscopy and DSC. Antimicrobial activity was tested in vitro on Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Sarcina lutea ATCC 9341), Gram-negative bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC), and fungus (Candida albicans ATCC 10231, Candida glabrata ATCC MYA 2950, Candida parapsilosis ATCC 22019). The inclusion complex showed better antibacterial effect for S. aureus and S. lutea versus thiotriazinone. It may be noted that the compounds tested showed no antibacterial activity for E. coli and Pseudomonas aeruginosa. Although the tested compounds show antifungal activity against all fungal organisms, a more pronounced action was observed especially against C. glabrata.
listelement.badge.access-status Open Access ,
Simuliosis – A dermatosis caused by black flies
(Elsevier B.V., 2016-01-04) Chiriac, Anca; Brzezinski, Piotr; Miron, Liviu-Dan; Moldovan, Cosmin; Podoleanu, Cristian; Stolnicu, Simona
listelement.badge.access-status Metadata only ,
Heat treatment of serum samples from stray dogs naturally exposed to Dirofilaria immitis and Dirofilaria repens in Romania
(Elsevier B.V., 2016-06-01) Ciucă, Lavinia; Genchi, Marco; Kramer, Laura; Mangia, C.; Miron, Liviu-Dan; Del Prete, L.; Maurelli, Maria-Paola; Cringoli, Giuseppe; Rinaldi, Laura
Pre-heating of serum samples has been shown to reverse false negative antigen tests for Dirofilaria immitis infection in dogs. Here the authors report the results of serum sampling in a population of dogs naturally exposed to D. immitis and Dirofilaria repens infection by testing in ELISA before and after heat treatment. Of 194 dogs sampled from four cities in Romania, D. immitis circulating antigens were found in 16 (8.2%) non heated samples and in 52 (26.8%) heated samples. Of the 108 dogs examined by Knott test, 24 dogs (22.2%) were positive for circulating mf. Subsequent PCR identification showed six dogs had D. immitis mf only, 12 dogs, had only D. repens mf, and 5 were positive for both. Fifty% of dogs with circulating D. immitis mf had positive antigen tests before and after heating, while the other 50% reverted to positive only after heat treatment. Sixty% of dogs with mixed D. immitis/D. repens infection were antigen positive before and after heating, while the other 40% converted to positive after heating. Antigen testing for D. immitis in the 12 dogs with only D. repens mf gave conflicting results. Only two dogs (16%) were antigen negative both before and after heat treatment. Six dogs (50%) became antigen positive after heating and four dogs (30%) were antigen positive both before and after heat treatment. Results would suggest that: false negative result for antigen testing can be reverted by heating of the serum sample; dogs infected with D. repens may have also an occult infection with D. immitis; heat treatment of serum from D. repens-infected dogs can reveal an occult infection with D. immitis.
listelement.badge.access-status Open Access ,
Climate change is increasing the risk of the reemergence of malaria in Romania
(Hindawi Publishing Corporation, 2016-08-07) Ivănescu, Maria-Larisa; Bodale, Ilie; Florescu, Simin-Aysel; Roman, Constantin; Acatrinei, Dumitru-Mihai; Miron, Liviu-Dan
The climatic modifications lead to global warming; favouring the risk of the appearance and development of diseases are considered until now tropical diseases. Another important factor is the workers’ immigration, the economic crisis favouring the passive transmission of new species of culicidae from different areas. Malaria is the disease with the widest distribution in the globe. Millions of people are infected every year in Africa, India, South-East Asia, Middle East, and Central and South America, with more than41% of the global population under the risk of infestation with malaria. The increase of the number of local cases reported in 2007–2011 indicates that the conditions can favour the high local transmission in the affected areas. In the situation presented, theestablishment of the level of risk concerning the reemergence of malaria in Romania becomes a priority.
listelement.badge.access-status Open Access ,
Geographic distribution of canine heartworm (Dirofilaria immitis) infection in stray dogs of Eastern Romania
(Licensee PAGEPress, Italy, 2018-08-03) Ciucă, Lavinia; Musella, Vincenzo; Miron, Liviu-Dan; Maurelli, Maria-Paola; Cringoli, Giuseppe; Bosco, Antonio; Rinaldi, Laura
A survey was conducted in the eastern part of Romania to assess theprevalence and geographical distribution of Dirofilaria immitisindogs. Plasma samples were collected from 458 stray dogs hosted inshelters in 8 counties and tested serologically for the presence ofheartworm. In addition, 45 blood samples from dogs of a shelter inGalati city were examined by the modified Knott and multiplex poly-merase chain reaction (PCR) techniques. The immmunological assayshowed a heartworm infection prevalence of 8.9% in the dogs. Opticaldensity results for positive samples ranged between 0.217 and 2.683.Geographical information systems (GIS) were used to produce over-lays of distribution maps of D. immitisprevalence and predictive mapsbased on temperature suitability. High prevalence of D. immitiswasfound in the central East up to the northern border of the country, i.e.Galati county (60%), followed by the counties of Vaslui (12.0%) andIasi (7.7%). Out of 45 samples examined using the Knott test, 23 werepositive for circulating microfilariae (51.1%), while 19 dogs were pos-itive for D. immitisand 4 for both D. immitis andD. repens with themultiplex PCR test. The high prevalence for D. immitisshown in dogsin the Southeast (Galati, 42.2%) also by multiplex PCR gave strongsupport to the results achieved by the serological tests. The presentstudy confirms the ability of GIS to predict the distribution and epi-demiology of dirofilariosis in different geographical territories as hasbeen already demonstrated by the empirical epidemiological dataobtained at the continental, national and intraregional levels.

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