2014

Permanent URI for this collectionhttps://repository.iuls.ro/handle/20.500.12811/5111

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Investigations in associated protozoa-bacterial infections of cyprinids from a fish farm situated on the Jijia river in N-E of Romania
(Universidade Federal de Minas Gerais, Escola de Veterinária, 2014-06-01) Lazăr, M.; Miron, Liviu-Dan; Gostin, I.; Rîmbu, C.; Lazăr, R.; Guguianu, Eleonora
In autumn 2011 in cyprinid farms located in Iasi on the Jijia river, several infections with bacterial strains and macroscopical external cysts on the skin were diagnosed, which developed as a result of the stress induced by biotic and abiotic factors. On the examination of the cyst contents the presence of numerous spores was observed, mostly of the Dermocystidium sp genus. The samples were taken from the common carp (Cyprinus carpio) and crucian carp (Carassius auratus gibelio) species from the fish farm as well as from the Jijia River. 35 fish were examined, all of them showing cysts, fragmentation of their dorsal fin and congestion of the gills. Histological examination of the skin showed a field of multiple dermal cysts with round light eosinophilic formations (14-16μm) containing a central refractable body similar to that reported for Dermocystidium sp. Gills samples were taken from the affected areas for the SEM examination with the purpose of evaluating not only aspects of normal morphology, but also aspects of some modifications of the affected areas as well as the presence of the etiologically incriminated bacteria Pseudomonas fluorescens. The isolates were identified through phenotypic methods. All the strains that showed mobility and oxidase-positivity were tested using API 20 NE strip. Consequently, they were taxonomically grouped into the species Pseudomonas fluorescens. The scanning electron microscope (SEM) was used for the first time in the characterization of the bacterial lesions produced by Pseudomonas strains on Cyprinus carpio and Carassius auratus gibelio gills. The diagnosis of septicemia with conditional pathogen species of Pseudomonas fluorescens was correlated with the results of the physico-chemical investigations of water and the data concerning the breeding conditions of the investigated livestock.
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Coprological investigations on strongyle EPG profiles in working horses and horses residing in stud farms in Romania
(Elsevier, 2014-09-03) Buzatu, Marius-Cătălin; Mitrea, Ioan-Liviu; Miron, Liviu-Dan; Ioniță, Mariana
Nematodes belonging to the group of equine strongyles have a huge impact on equine health, as infection can lead to weight-loss and unthriftiness, disease symptoms dominated by colic and diarrhea, and death in severe cases. A new concept in the control of these parasites is presently addressed, toward to new strategies based on thorough strongyle surveillance and minimal application of anthelmintic treatment. The aim of such approach is to reserve treatments for horses in need for this, and thereby delay further development of resistance. Identification of high egg shedders within the herd is an essential goal. Therefore, the present study aimed to establish strongyle EPG profiles for working horses (n = 142) and horses residing in studs (n = 138) in Romania, based on the strongyle eggs per gram of feces (EPG) counts. All horses aged over 1 year. Fecal samples of 69.01% and 86.23% working horses and horses residing in studs, respectively, were positive for strongyle. Of them, 44.44% of the working horses and 72.5% of horses from studs exceeded the cut-off value of 250 strongyle EPG. This research showed the importance of strongyle EPG profiling for horses as base for further studies in designing and monitoring sustainable control program of equine parasites.

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